Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-194) antibody

Antigen: Signal Transducer and Activator of Transcription 1, 91kDa (STAT1)

Synonyms: ISGF-3, STAT91, DKFZp686B04100, AA408197, 2010005J02Rik, IMUP, H2RSP, IMUP-1, IMUP-2

Binding Site: AA 1-194

Clonality: Monoclonal (1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967759

Additional Information

Alternative name: Stat1

Immunogen: Human Stat1

Cross-Reactivity: Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 1

Description: Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84 kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Thus, phosphorylation of Y701 in Stat1 occurs in response to growth factors and cytokines, and is essential for normal transcriptional activity of the ISGF3 complex. The 1/Stat1 monoclonal antibody recognizes the N-terminus of human Stat1 (both isoforms), regardless of phosphorylation status. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 91/84 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Stat1 on a A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Stat1 antibody.

Immunofluorescent staining of human fibroblast cells.

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-194) antibody (Image 3)

Publications

Product: Fu, Schindler, Improta et al.: "The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family involved in signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7840-3, 1992 (PubMed).

Sadowski, Shuai, Darnell et al.: "A common nuclear signal transduction pathway activated by growth factor and cytokine receptors." in: Science (New York, N.Y.), Vol. 261, Issue 5129, pp. 1739-44, 1993 (PubMed).

Dumoutier, Louahed, Renauld: "Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1814-9, 2000 (PubMed).

Doan, Ali, Bernstein: "Tyrosine kinase activation by the angiotensin II receptor in the absence of calcium signaling." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 20954-8, 2001 (PubMed).

Dupuis, Dargemont, Fieschi et al.: "Impairment of mycobacterial but not viral immunity by a germline human STAT1 mutation." in: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 300-3, 2001 (PubMed).