Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody

Details for Product No. ABIN968779
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Antigen
Synonyms IMUP, H2RSP, IMUP-1, IMUP-2, 2010005J02Rik, AA408197, DD6G4-4, CANDF7, ISGF-3, STAT91, STAT4, XStat1, STAT, STAT1, dZ182H3.3, si:by134g18.2, si:by51f19.1, si:dz182h3.3, si:dz199m19.1, si:dz73o4.2
Epitope
pTyr701
(54), (51), (48), (14), (9), (9), (9), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(311), (165), (150), (43), (41), (29), (27), (4), (2), (1), (1)
Host
Mouse
(260), (62), (4), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(291), (113), (97), (76), (66), (30), (28), (27), (7), (4), (4), (2), (1), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN968779
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Immunogen Phosphorylated Human Stat1 Peptide
Clone 4a
Isotype IgG2a
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Stat1
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs).
The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
Molecular Weight 84/91 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody A431 cells were either left untreated (left lane) or treated (right lane) with 100 ng/ml EGF for 5 minutes at 37°C. The top panel was probed with Stat1 (ABIN967758) and the bottom was probed with Stat1 (pY701) (ABIN968779).
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (2) A431 cells were serum starved and treated with EGF (100 ng/ml) for 5 minutes, then fixed in 3.75% paraformaldehyde with 0.2% Triton X-100. Immunofluorescent staining was performed with Stat1 (ABIN967758) and Stat1 (pY701) (ABIN968779).
Product cited in: Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993 (PubMed).

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997 (PubMed).

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