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UHRF1 antibody (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1) (AA 199-298)

Details for Product anti-UHRF1 Antibody No. ABIN968794, Supplier: Log in to see
Antigen
  • UHRF1
  • ICBP90
  • Np95
  • RNF106
  • hNP95
  • hUHRF1
  • NP95
  • fb97f09
  • wu:fb97f09
  • zgc:63539
  • AL022808
  • C6orf107
  • dJ349A12.1
  • ubiquitin-like with PHD and ring finger domains 1
  • ubiquitin-like, containing PHD and RING finger domains, 1
  • UHRF1 binding protein 1
  • UHRF1
  • Uhrf1
  • uhrf1
  • UHRF1BP1
Epitope
AA 199-298
16
14
12
10
5
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Human
92
9
4
3
2
2
1
1
1
Host
Mouse
66
22
10
1
Clonality (Clone)
Monoclonal ()
Conjugate
This UHRF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Fluorescence Microscopy (FM), Western Blotting (WB)
89
46
33
18
13
11
9
8
7
2
1
1
Options
Supplier
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Immunogen Human ICBP90 aa. 199-298
Clone 28-ICBP90
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ICBP90 (UHRF1 Antibody Abstract)
Background DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA. Topoisomerase I produces transient single-stranded breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA. These DNA breaks are important for DNA replication and chromosome condensation and segregation. Two types of topoisomerase II have been identified, TopoIIalpha and TopoIIbeta. During interphase, TopoIIalpha is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIalpha is found associated with metaphase chromosomes and centromeres. Transcriptional control of TopoIIalpha expression is regulated through multiple gene promoters, which include five inverted CCAAT boxes, one ATF binding sequence, and two GC-rich boxes. ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted CCAAT box. ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow. Overexpression of ICBP90 in COS-1-transfected cells leads to enhanced expression of TopoIIalpha. ICBP90 co-localizes with TopoIIalpha in proliferating areas of appendix and in high grade breast carcinomas. Thus, ICBP90 may be an important transcripitonal regulator of TopoIIalpha expression in proliferating cells.
Molecular Weight 97 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-UHRF1 antibody (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1) (AA 199-298) (ABIN968794) Western blot analysis of ICBP90 on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3:...
Product cited in: Hopfner, Mousli, Garnier, Redon, du Manoir, Chatton, Ghyselinck, Oudet, Bronner: "Genomic structure and chromosomal mapping of the gene coding for ICBP90, a protein involved in the regulation of the topoisomerase IIalpha gene expression." in: Gene, Vol. 266, Issue 1-2, pp. 15-23, 2001 (PubMed).

Hopfner, Mousli, Jeltsch, Voulgaris, Lutz, Marin, Bellocq, Oudet, Bronner: "ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression." in: Cancer research, Vol. 60, Issue 1, pp. 121-8, 2000 (PubMed).