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Smooth Muscle Actin antibody (Actin, alpha 2, Smooth Muscle, Aorta)

Details for Product anti-ACTA2 Antibody No. ABIN968945, Supplier: Log in to see
Antigen
  • 0610041G09Rik
  • a-sma
  • a-SMA
  • aat6
  • AAT6
  • ACT-4
  • ACTA2
  • actc1
  • ACTSA
  • actsa
  • Actvs
  • alphaSMA
  • ASMA
  • asma
  • MYMY5
  • mymy5
  • sm-actin
  • SMactin
  • SMalphaA
  • wu:fb63d03
Reactivity
Human, Monkey, Rat (Rattus), Mouse (Murine)
204
73
65
26
18
16
15
13
8
6
5
5
4
3
3
2
2
1
1
Host
Mouse
104
90
15
Clonality (Clone)
Monoclonal ()
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
9
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
125
104
64
59
39
30
14
13
4
3
1
1
Supplier
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Immunogen Purified recombinant fragment of human ACTA2 expressed in E. coli.
Clone 1H8
Isotype IgG1
Purification Ascites
Alternative Name ACTA2 (ACTA2 Antibody Abstract)
Background Actin,alpha 2, smooth muscle, aorta, major constituent of thin filaments.
Molecular Weight 42 kDa
Gene ID 59
HGNC 59
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Smooth Muscle Actin antibody (Actin, alpha 2, Smooth Muscle, Aorta) (ABIN968945) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Smooth Muscle Actin antibody (Actin, alpha 2, Smooth Muscle, Aorta) (ABIN968945) Figure 2: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-Smooth Muscle Actin antibody (Actin, alpha 2, Smooth Muscle, Aorta) (ABIN968945) Figure 3: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green) ...
Western Blotting (WB) image for anti-Smooth Muscle Actin antibody (Actin, alpha 2, Smooth Muscle, Aorta) (ABIN968945) Figure 4: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), H...
Product cited in: Guo, Pannu, Tran-Fadulu, Papke, Yu, Avidan, Bourgeois, Estrera, Safi, Sparks, Amor, Ades, McConnell, Willoughby, Abuelo, Willing, Lewis, Kim, Scherer, Tung, Ahn, Buja, Raman, Shete, Milewicz: "Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections." in: Nature genetics, Vol. 39, Issue 12, pp. 1488-93, 2007

Salas, Bartoli, Deville, Gaudart, Fina, Calisti, Bollini, Curvale, Gentet, Duffaud, Figarella-Branger, Bouvier: "Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas." in: Virchows Archiv : an international journal of pathology, Vol. 451, Issue 6, pp. 999-1007, 2007