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anti-Human CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Monkey
611
461
142
67
25
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Mouse
476
411
113
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
163
123
87
51
18
17
12
12
11
11
11
10
9
9
7
6
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
797
242
205
159
136
111
78
75
71
67
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
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Catalog No. ABIN118516
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008774526 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
0.0008774526 ABIN238453 Func FACS Mouse IgG1 Log in to see MEM-170 3
0.0008774526 ABIN192410 Func FACS Mouse IgG1 Log in to see MEM-170 3
0.0008774526 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
0.0008774526 ABIN457402 Func FACS IHC (fro) IP Rat IgG2b Log in to see M1-70 5
0.0008774526 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
0.0008774526 ABIN1027692 Func ICC IHC (fro) FACS IP Mouse IgG1 Log in to see ICRF44 3
0.0008774526 ABIN1690733 Func IHC (fro) FACS IP APC Rat IgG2b Log in to see M1-70 10
0.0008774526 ABIN126001 Func FACS IP Mouse IgG2a Log in to see MEM-174
0.0008774526 ABIN609928 Func FACS FITC Mouse IgG1 Log in to see ICR44
0.0008774526 ABIN609927 Func FACS Biotin Mouse IgG1 Log in to see ICR44
0.0008774526 ABIN609929 Func FACS PE Mouse IgG1 Log in to see ICR44
0.0008774526 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 Log in to see ICRF44 4
0.0008774526 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3
0.0008774526 ABIN1176910 Func FACS Mouse IgG1, kappa Log in to see ICRF44
0.0008774526 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4
0.0008774526 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 Log in to see ICRF44 4
0.0008774526 ABIN280223 Func FACS IHC (fro) IHC Mouse IgG1 kappa Log in to see ICRF44
0.0008774526 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
0.0008774526 ABIN206917 Func FACS Mouse IgG1 Log in to see MEM-170

Top referenced anti-CD11b antibody for Functional Studies

Similar anti-CD11b Antibodies

Application / Reactivity Monkey Human
Flow Cytometry (FACS) 26 Antibodies 446 Antibodies
Functional Studies (Func) 20 Antibodies 60 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 60 Antibodies
Immunofluorescence (IF) 5 Antibodies 92 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 11 Antibodies
ELISA 62 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Monkey
(611), (461), (142), (67), (25), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Mouse
(476), (411), (113), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(163), (123), (87), (51), (18), (17), (12), (12), (11), (11), (11), (10), (9), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(797), (242), (205), (159), (136), (111), (78), (75), (71), (67), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118516) Images
Specificity This antibody recognizes the CD11b cell surface glycoprotein. Clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.
No Cross-Reactivity Cat (Feline)
Cross-Reactivity (Details) Species reactivity (tested):Human, Cynomolgus Monkey , Rhesus Monkey, Baboon.
Purification Affinity Chromatography on Protein G
Immunogen Rheumatoid synovial cells and Human monocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Clone ICRF44
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN118516) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is a 165 kD molecule also know as the alpha-M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta-2 integrin and is found on monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. ICR44 significantly blocks homotypic PNM aggregation on response to FMLP (3,4).Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
NCBI Accession NP_000623
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN118516) Images back to top
Application Notes Flow Cytometry (use 10 μL of 1:10 - 1:100 diluted antibody to label 10^6 cells or 100 μL whole blood). Immunoprecipitation Immunohistochemistry on frozen sections (1:100 - 1:1000) Recommended positive control tissue: Human tonsil. Not suitable for paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN118516) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.

References for anti-CD11b Antibody (ABIN118516)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Ramacciotti, Blackburn, Hawley, Vandy, Ballard-Lipka, Stabler, Baker, Guire, Rectenwald, Henke, Myers, Wakefield: "Evaluation of soluble P-selectin as a marker for the diagnosis of deep venous thrombosis." in: Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, Vol. 17, Issue 4, pp. 425-31, 2011

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, 1992

Malhotra, Hogg, Sim: "Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3)." in: European journal of immunology, Vol. 16, Issue 9, pp. 1117-23, 1986

Product cited in:

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, 2006

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118516) back to top
Supplier Images
 image for anti-CD11b antibody (Integrin alpha M) (ABIN118516) Staining of human peripheral blood granulocytes with Mouse anti Human CD11b (ABIN1185...