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anti-Human CD11b antibody for Intracellular Staining

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human
648
492
202
65
26
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Mouse
492
398
161
18
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to FITC
166
128
94
51
16
15
15
15
14
13
11
11
9
9
6
6
6
6
6
5
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
852
239
210
169
149
148
100
98
86
71
26
19
17
13
8
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002766
$ 385.77
Plus shipping costs $45.00

Similar anti-CD11b Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 11 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 492 Antibodies
Functional Studies (Func) 61 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 90 Antibodies
Immunofluorescence (IF) 123 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 116 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 106 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 78 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human
(648), (492), (202), (65), (26), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(492), (398), (161), (18), (4), (2)
Clonality
Monoclonal
Conjugate This CD11b antibody is conjugated to FITC
(166), (128), (94), (51), (16), (15), (15), (15), (14), (13), (11), (11), (9), (9), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
(852), (239), (210), (169), (149), (148), (100), (98), (86), (71), (26), (19), (17), (13), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling Images
Specificity Specifically recognizes human Mac-1. LM2/1 binds to the I domain of MAC-1 subunit (CD11b).
No Cross-Reactivity There are no data about cross-reactivity with other species.
Purity > 95 % IgG1 as determined by SDS-gel electrophoresis.
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mac-1 alpha (CD11b) (ITGAM Antibody Abstract)
Background Mouse Anti-Human Macrophage Antigen 1-alpha (CD11b, FITC-labeled)
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling Images back to top
Application Notes Immunohistochemistry: ABIN1002766 can be used to stain acetone-fixed cryostat sections or cell smears. Recommended for ABIN1002766 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. ABIN1002766 may be used at a concentration of 0.5 - 5 µg/mL.Flow cytometry:ABIN1002766 is also suitable as primary antibody in staining for FACS analysis, ABIN1002766 (FITC-labeled antibody) can be used for direct staining of cells, ABIN1002766 (biotinylated antibody) can be used in a two-step procedure using the biotin-(strept)avidin system. Recommended dilution is 1:20 as determined on RPMI8866 cells (for exact concentration see vial label).Optimal antibody concentrations should be determined by the individual laboratory for each application.This antibody is also available as biotinylated conjugate.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details Images back to top
Format Liquid
Buffer 50 mM TRIS ,100 mM NaCl ,1% BSA ,0.02% NaN₃, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment 2-8°C until expiry as indicated on label. After opening aliquote contents and freeze at -20°C
Expiry Date 12-14 months