anti-Human CD11b antibody for Intracellular Staining

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human
704
492
192
78
31
19
17
15
13
11
9
9
7
7
5
4
4
4
2
2
Host
Mouse
507
388
166
16
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to FITC
151
124
92
52
20
19
15
15
14
14
14
13
11
10
9
9
9
8
7
7
7
6
6
6
6
6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
878
234
228
173
170
138
98
97
95
67
25
21
21
13
12
8
5
5
4
3
3
1
1
1
1
1
1
1
1
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Catalog No. ABIN1002766
$ 385.77
Plus shipping costs $45.00

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human
(704), (492), (192), (78), (31), (19), (17), (15), (13), (11), (9), (9), (7), (7), (5), (4), (4), (4), (2), (2)
Host Mouse
(507), (388), (166), (16), (6), (2)
Clonality
Monoclonal
Conjugate This CD11b antibody is conjugated to FITC
(151), (124), (92), (52), (20), (19), (15), (15), (14), (14), (14), (13), (11), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
(878), (234), (228), (173), (170), (138), (98), (97), (95), (67), (25), (21), (21), (13), (12), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling Images
Specificity Specifically recognizes human Mac-1. LM2/1 binds to the I domain of MAC-1 subunit (CD11b).
No Cross-Reactivity There are no data about cross-reactivity with other species.
Purity > 95 % IgG1 as determined by SDS-gel electrophoresis.
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mac-1 alpha (CD11b) (ITGAM Antibody Abstract)
Background Mouse Anti-Human Macrophage Antigen 1-alpha (CD11b, FITC-labeled)
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling Images back to top
Application Notes Immunohistochemistry: ABIN1002766 can be used to stain acetone-fixed cryostat sections or cell smears. Recommended for ABIN1002766 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. ABIN1002766 may be used at a concentration of 0.5 - 5 µg/mL.Flow cytometry:ABIN1002766 is also suitable as primary antibody in staining for FACS analysis, ABIN1002766 (FITC-labeled antibody) can be used for direct staining of cells, ABIN1002766 (biotinylated antibody) can be used in a two-step procedure using the biotin-(strept)avidin system. Recommended dilution is 1:20 as determined on RPMI8866 cells (for exact concentration see vial label).Optimal antibody concentrations should be determined by the individual laboratory for each application.This antibody is also available as biotinylated conjugate.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details Images back to top
Format Liquid
Buffer 50 mM TRIS ,100 mM NaCl ,1% BSA ,0.02% NaN₃, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment 2-8°C until expiry as indicated on label. After opening aliquote contents and freeze at -20°C
Expiry Date 12-14 months