anti-Human CD11b antibody for Immunoprecipitation

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human
732
515
192
79
31
19
17
15
13
11
10
9
8
7
5
4
4
4
4
2
Host
Mouse
532
410
168
17
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
150
128
94
56
24
24
20
17
15
14
14
13
13
13
11
11
11
10
9
7
7
6
6
6
6
6
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
930
237
235
173
172
138
100
98
97
68
25
21
21
18
14
8
5
5
4
3
3
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN125683
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.04621 ABIN1302538 FACS IP APC Mouse IgG2a Log in to see MEM-174 4
24.04621 ABIN93959 FACS IP FITC Mouse IgG2a Log in to see MEM-174 5
24.04621 ABIN93960 FACS IP PE Mouse IgG2a Log in to see MEM-174 5
24.04621 ABIN93956 FACS IP Mouse IgG2a Log in to see MEM-174 5
24.04621 ABIN93958 FACS IP Biotin Mouse IgG2a Log in to see MEM-174 5
1 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
1 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
1 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
1 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
1 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
1 ABIN2659829 FACS IP PerCP Rat IgG2b kappa Log in to see M1-70 11
1 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
1 ABIN573960 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN4620335 IHC (fro) IF IP Mouse IgG1 Log in to see Bear-1 2
1 ABIN2662970 BR FACS IP IHC WB PE Mouse IgG1 kappa Log in to see ICRF44 7
1 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
1 ABIN2478219 IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human
(732), (515), (192), (79), (31), (19), (17), (15), (13), (11), (10), (9), (8), (7), (5), (4), (4), (4), (4), (2)
Host Mouse
(532), (410), (168), (17), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(150), (128), (94), (56), (24), (24), (20), (17), (15), (14), (14), (13), (13), (13), (11), (11), (11), (10), (9), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(930), (237), (235), (173), (172), (138), (100), (98), (97), (68), (25), (21), (21), (18), (14), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN125683) Images
Specificity The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI, WS Code BP 310
HLDA VI, WS Code M 18
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Human granulocytes
Clone MEM-174
Isotype IgG2a

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN125683) Images back to top
Antigen
Alternative Name ITGAM (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN125683) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN125683) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD11b antibody (ABIN125683)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Background publications

Lawrence, Srikumaran: "CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization." in: Veterinary immunology and immunopathology, Vol. 119, Issue 3-4, pp. 287-98, 2007

Akramiene, Kondrotas, Didziapetriene, Kevelaitis: "Effects of beta-glucans on the immune system." in: Medicina (Kaunas, Lithuania), Vol. 43, Issue 8, pp. 597-606, 2007

Hentzen, Neelamegham, Kansas, Benanti, McIntire, Smith, Simon: "Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1." in: Blood, Vol. 95, Issue 3, pp. 911-20, 2000

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN125683) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Integrin alpha M (ITGAM) antibody (ABIN125683) Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC is...