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anti-Human CD11b antibody for Immunoprecipitation

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human
611
461
142
67
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Mouse
476
411
113
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to APC
163
123
87
50
18
17
12
12
11
11
11
10
9
9
7
6
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
797
242
205
160
136
111
78
75
72
67
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
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Catalog No. ABIN1302538
$ 334.95
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.51158696 ABIN93956 FACS IP Mouse IgG2a Log in to see MEM-174 5
0.51158696 ABIN93959 FACS IP FITC Mouse IgG2a Log in to see MEM-174 5
0.51158696 ABIN125683 FACS IP Mouse IgG2a Log in to see MEM-174 5
0.00089459505 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
0.00089459505 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
0.00089459505 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
0.00089459505 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
0.00089459505 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
0.00089459505 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
0.00089459505 ABIN1105776 IHC (fro) IF IP Mouse IgG1 Log in to see Bear-1 2
0.00089459505 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
0.00089459505 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
0.00089459505 ABIN616777 IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 13
0.00089459505 ABIN616776 IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 13
0.00089459505 ABIN2659829 FACS IP PerCP Rat IgG2b kappa Log in to see M1-70 11
0.00089459505 ABIN457402 Func FACS IHC (fro) IP Rat IgG2b Log in to see M1-70 5
0.00089459505 ABIN2662970 BR FACS IP IHC WB PE Mouse IgG1 kappa Log in to see ICRF44 7
0.00089459505 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
0.00089459505 ABIN1027692 Func ICC IHC (fro) FACS IP Mouse IgG1 Log in to see ICRF44 3
0.00089459505 ABIN120554 FACS IP Mouse IgG2a Log in to see MEM-174

Top referenced anti-CD11b antibody for Immunoprecipitation

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 11 Antibodies
ELISA 62 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 446 Antibodies
Functional Studies (Func) 60 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 60 Antibodies
Immunofluorescence (IF) 92 Antibodies
Immunohistochemistry (IHC) 121 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 95 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 57 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human
(611), (461), (142), (67), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Mouse
(476), (411), (113), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to APC
(163), (123), (87), (50), (18), (17), (12), (12), (11), (11), (11), (10), (9), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(797), (242), (205), (160), (136), (111), (78), (75), (72), (67), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN1302538) Images
Specificity The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.HLDA VI, WS Code BP 310HLDA VI, WS Code M 18
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Immunogen Human granulocytes
Clone MEM-174
Isotype IgG2a

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN1302538) Images back to top
Antigen
Alternative Name ITGAM (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages.CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Gene ID 3684
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN1302538) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens (Human)

Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN1302538) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

References for anti-CD11b Antibody (ABIN1302538)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Background publications

Lawrence, Srikumaran: "CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization." in: Veterinary immunology and immunopathology, Vol. 119, Issue 3-4, pp. 287-98, 2007

Akramiene, Kondrotas, Didziapetriene, Kevelaitis: "Effects of beta-glucans on the immune system." in: Medicina (Kaunas, Lithuania), Vol. 43, Issue 8, pp. 597-606, 2007

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN1302538) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-CD11b antibody (Integrin alpha M)  (APC) (ABIN1302538) Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC is...