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anti-Mouse (Murine) CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Mouse (Murine), Rabbit
634
484
196
65
26
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Rat
490
394
154
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PE
166
127
93
51
16
15
15
14
14
13
11
11
9
9
7
6
6
6
6
5
5
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
823
249
221
162
158
123
98
80
75
71
24
19
17
13
8
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN118147
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28290245 ABIN118142 FACS FITC Rat IgG2b Log in to see M1-70-15 7
0.28290245 ABIN118143 FACS FITC Rat IgG2b Log in to see M1-70-15 6
0.28290245 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
0.28290245 ABIN258322 ICC FACS IF IHC (p) IHC WB Rabbit Internal Region, AA 500-600 Log in to see Polyclonal 16
0.28290245 ABIN370850 FACS IHC (fro) IP Rat IgG2a Log in to see 3A33 11
0.28290245 ABIN2656815 FACS Alexa Fluor 594 Rat IgG2b kappa Log in to see M1-70 7
0.28290245 ABIN2657615 FACS Alexa Fluor 647 Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2657315 FACS IHC Alexa Fluor 488 Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2661310 FACS Biotin Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2658847 FACS APC Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2658907 FACS PE-Cy5 Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2659829 FACS IP PerCP Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2661484 FACS FITC Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2663957 FACS PE Rat IgG2b kappa Log in to see M1-70 8
0.28290245 ABIN2659423 FACS PE-Cy7 Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2660632 FACS APC-Cy7 Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2662110 FACS Pacific Blue Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2665948 FACS IF Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN2664164 FACS Rat IgG2b kappa Log in to see M1-70 11
0.28290245 ABIN439874 FACS ICC IHC IHC (fro) IHC (p) IP in vitro FITC Rat IgG2b kappa Log in to see M1-70

Top referenced anti-CD11b antibody for Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Human Mouse (Murine) Rabbit
Simple Western (SimWes) 1 Antibodies 1 Antibodies
Western Blotting (WB) 140 Antibodies 75 Antibodies 2 Antibodies
Staining Methods (StM) 2 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 6 Antibodies 4 Antibodies
In vitro Assay (in vitro) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 129 Antibodies 125 Antibodies 42 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine), Rabbit
(634), (484), (196), (65), (26), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Rat
(490), (394), (154), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PE
(166), (127), (93), (51), (16), (15), (15), (14), (14), (13), (11), (11), (9), (9), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(823), (249), (221), (162), (158), (123), (98), (80), (75), (71), (24), (19), (17), (13), (8), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118147) Images
Purification Affinity Chromatography on Protein G
Immunogen T cell enriched splenocytes from B10 mice. Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Clone M1-70-15
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN118147) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN118147) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN118147) Images back to top
Reconstitution Restore with distilled water.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

References for anti-CD11b Antibody (ABIN118147)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Klingel, Schnorr, Sauter, Szalay, Kandolf: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118147) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M)  (PE) (ABIN118147) Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophag...