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anti-Non-Human Primate CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Non-Human Primate, Mouse (Murine), Rabbit
615
466
146
66
26
20
17
17
17
11
10
8
5
4
3
2
2
1
Host
Rat
477
412
117
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
164
123
88
51
18
17
12
12
11
11
11
10
10
9
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
799
243
207
161
140
112
78
77
71
69
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN457402
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008771965 ABIN1027692 Func ICC IHC (fro) FACS IP Mouse IgG1 Log in to see ICRF44 3
0.0008771965 ABIN1690733 Func IHC (fro) FACS IP APC Rat IgG2b Log in to see M1-70 10
0.0008771965 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 Log in to see ICRF44 4
0.0008771965 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3
0.0008771965 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4
0.0008771965 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 Log in to see ICRF44 4

Top referenced anti-CD11b antibody for Functional Studies

Similar anti-CD11b Antibodies

Application / Reactivity Rabbit Non-Human Primate Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies
Flow Cytometry (FACS) 66 Antibodies 9 Antibodies
Functional Studies (Func) 12 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 19 Antibodies 5 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunoprecipitation (IP) 41 Antibodies 7 Antibodies
Western Blotting (WB) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 4 Antibodies
Biochemical Assay (BCA) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Non-Human Primate, Mouse (Murine), Rabbit
(615), (466), (146), (66), (26), (20), (17), (17), (17), (11), (10), (8), (5), (4), (3), (2), (2), (1)
Host Rat
(477), (412), (117), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(164), (123), (88), (51), (18), (17), (12), (12), (11), (11), (11), (10), (10), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(799), (243), (207), (161), (140), (112), (78), (77), (71), (69), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images
Specificity The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Purification Purified from cell culture supernatant by protein-G affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen B10 mouse spleen cells enriched for T cells
Clone M1-70
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
Antigen
Alternative Name ITGAM (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1 µg/mL
Functional Application: In vitro blocking of CD11b

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN457402) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD11b Antibody (ABIN457402)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003

Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, 2000

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981