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anti-Rat (Rattus) CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Rat (Rattus)
649
492
201
65
26
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Mouse
492
398
161
18
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
167
128
94
51
16
15
15
15
14
13
11
11
9
9
6
6
6
6
6
5
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
852
238
211
168
149
148
100
97
86
70
26
19
17
13
8
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120414
$ 720.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.102535 ABIN120410 FACS IHC (fro) Biotin Mouse IgG2a Log in to see OX-42 3
13.102535 ABIN258322 ICC FACS IF IHC (p) IHC WB Rabbit Internal Region, AA 500-600 Log in to see Polyclonal 16
13.102535 ABIN730918 FACS IF (p) IHC (p) WB Rabbit IgG AA 50-100 Log in to see Polyclonal 7
13.102535 ABIN741285 FACS IF (p) IHC (p) WB Rabbit IgG AA 520-570 Log in to see Polyclonal 4
13.102535 ABIN2661485 FACS FITC Mouse IgG2a kappa Log in to see OX-42 6
13.102535 ABIN2662974 Agon FACS IF IP PE Mouse IgG2a kappa Log in to see OX-42 6
13.102535 ABIN2658457 FACS APC Mouse IgG2a kappa Log in to see OX-42 6
13.102535 ABIN2657617 FACS Alexa Fluor 647 Mouse IgG2a kappa Log in to see OX-42 6
13.102535 ABIN2490245 FACS PE Mouse IgG2a Log in to see
13.102535 ABIN2490253 IHC (af) FACS IP Mouse IgG2a Log in to see
13.102535 ABIN133343 FACS Mouse IgG2a Log in to see OX-42
13.102535 ABIN2490248 FACS FITC Mouse IgG2a Log in to see
13.102535 ABIN2490247 FACS FITC Mouse IgG2a Log in to see
13.102535 ABIN2490251 IHC (af) FACS Biotin Mouse IgG2a Log in to see
13.102535 ABIN2490255 IHC (af) FACS IP Mouse IgG2a Log in to see
13.102535 ABIN2490254 IHC (af) FACS IP Mouse IgG2a Log in to see
13.102535 ABIN2490256 IHC (af) FACS IP Mouse IgG2a Log in to see
13.102535 ABIN2478198 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
1 ABIN409930 FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal 23
1 ABIN120413 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4

Top referenced anti-CD11b antibody for Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus)
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
ELISA 10 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 151 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Rat (Rattus)
(649), (492), (201), (65), (26), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(492), (398), (161), (18), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(167), (128), (94), (51), (16), (15), (15), (15), (14), (13), (11), (11), (9), (9), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(852), (238), (211), (168), (149), (148), (100), (97), (86), (70), (26), (19), (17), (13), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN120414) Images
Purification Affinity chromatography on Protein G
Immunogen Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Clone OX-42
Isotype IgG2a

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN120414) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10116
UniProt Q63001
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN120414) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN120414) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b Antibody (ABIN120414)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992

Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN120414) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M) (ABIN120414) Staining of rat peritoneal macrophages with MOUSE ANTI RAT CD11b(ABIN120414).