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anti-Rat (Rattus) CD11b antibody for Immunocytochemistry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Epitope
Internal Region, AA 250-350
20
19
14
14
8
6
6
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
569
431
133
48
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Rabbit
464
390
103
16
4
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
162
121
86
51
18
17
12
11
11
9
8
8
8
7
7
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
758
219
177
132
125
82
75
67
48
38
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Available images

Catalog No. ABIN409931
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010136499 ABIN1732800 FACS ICC IF IHC (p) WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal
0.0010136499 ABIN1859498 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 598-700 Log in to see Polyclonal
0.0010136499 ABIN1859499 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 870-1004 Log in to see Polyclonal
0.0010136499 ABIN1859494 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 31-160 Log in to see Polyclonal

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 8 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 80 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 28 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region, AA 250-350
(20), (19), (14), (14), (8), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(569), (431), (133), (48), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Rabbit
(464), (390), (103), (16), (4), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(162), (121), (86), (51), (18), (17), (12), (11), (11), (9), (8), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(758), (219), (177), (132), (125), (82), (75), (67), (48), (38), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling Images
Cross-Reactivity (Details) We have feedback that this antibody does not work in human samples with Western blot.
Purification affinity purified
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. Immunogen has 100% homology to bovine and 88% homology with sheep.Marker Microglia Marker, Myeloid Marke

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Gene Symbol: ITGAM
Gene ID 3684, 16409, 25021
UniProt P05555
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling Images back to top
Application Notes This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916)
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:400, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:400, Western Blot 2 µg/mL
Protocol Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details Images back to top
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409931) anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody