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anti-Rat (Rattus) CD11b antibody for Immunofluorescence

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Rat (Rattus)
552
431
132
48
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Mouse
446
390
103
16
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
162
120
85
51
18
17
12
11
11
8
7
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
757
218
177
131
108
81
75
66
47
38
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN120412
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN409931 ICC FACS IHC (fro) IF IHC (p) IHC WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal
0.0010850558 ABIN1732800 FACS ICC IF IHC (p) WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal
0.0010850558 ABIN2222348 FACS IF IP IHC Mouse IgG2a Log in to see 6A244
0.0010850558 ABIN2478198 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
0.0010850558 ABIN2478210 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4

Top referenced anti-CD11b antibody for Immunofluorescence

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 80 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 28 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Rat (Rattus)
(552), (431), (132), (48), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Mouse
(446), (390), (103), (16), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(162), (120), (85), (51), (18), (17), (12), (11), (11), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(757), (218), (177), (131), (108), (81), (75), (66), (47), (38), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN120412) Images
Purification Affinity chromatography on Protein G
Immunogen Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Clone OX-42
Isotype IgG2a

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN120412) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10116
UniProt Q63001
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN120412) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN120412) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b Antibody (ABIN120412)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992

Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981