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anti-Rat (Rattus) CD11b antibody for Immunohistochemistry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Epitope
Internal Region, AA 250-350
51
24
19
18
14
14
14
8
6
5
5
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
643
485
197
65
26
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Rabbit
494
394
156
18
4
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
166
128
94
51
16
15
15
15
14
13
11
11
9
9
6
6
6
6
6
5
5
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
843
232
203
175
148
147
104
99
93
71
22
19
17
13
8
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN409930
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.693808 ABIN258322 ICC FACS IF IHC (p) IHC WB Rabbit Internal Region, AA 500-600 Log in to see Polyclonal 16
8.693808 ABIN1584179 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 1
8.693808 ABIN2431615 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.693808 ABIN2426165 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN234503 FACS IP IHC Mouse IgG2a Log in to see OX-42 4
1 ABIN2664165 IF ICFC IHC Mouse IgG2a kappa Log in to see OX-42 6
1 ABIN3022180 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4289481 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN4289482 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN4289483 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN4289484 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN314167 ICC IF IHC IHC (p) WB Biotin Rabbit Internal Region, AA 500-600 Log in to see Polyclonal
1 ABIN4289476 FACS ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN4289478 FACS ICC IF IHC IHC (p) FITC Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN409442 FACS ICC IF IHC IHC (p) WB DyLight 488 Rabbit Internal Region, AA 500-600 Log in to see Polyclonal
1 ABIN4289477 FACS ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN4289480 FACS ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 500-600, Internal Region Log in to see Polyclonal
1 ABIN2222442 FACS IP IHC FITC Mouse IgG2a Log in to see 2Q913
1 ABIN2222430 FACS IHC Biotin Mouse IgG2a Log in to see 3H953
1 ABIN314168 IHC (p) IHC WB HRP Rabbit Internal Region, AA 500-600 Log in to see Polyclonal

Top referenced anti-CD11b antibody for Immunohistochemistry

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus)
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 145 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 46 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 31 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region, AA 250-350
(51), (24), (19), (18), (14), (14), (14), (8), (6), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(643), (485), (197), (65), (26), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(494), (394), (156), (18), (4), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(166), (128), (94), (51), (16), (15), (15), (15), (14), (13), (11), (11), (9), (9), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(843), (232), (203), (175), (148), (147), (104), (99), (93), (71), (22), (19), (17), (13), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409930) Images
Cross-Reactivity (Details) We have feedback that this antibody does not work in human samples with Western blot.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN409930) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Gene Symbol: ITGAM
Molecular Weight Theoretical MW: 127 kDa
Gene ID 3684
UniProt P11215, P05555
Research Area Stem Cells, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens, Glia marker, Hematopoietic Stem Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN409930) Images back to top
Application Notes Western Blot 2 μg/mL, Simple Western 1:50, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry-Paraffin 1:400, Immunohistochemistry-FrozenThis CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN409930) Images back to top
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-CD11b Antibody (ABIN409930)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Algieri, Rodriguez-Nogales, Garrido-Mesa, Camuesco, Vezza, Garrido-Mesa, Utrilla, Rodriguez-Cabezas, Pischel, Galvez: "Intestinal anti-inflammatory activity of calcium pyruvate in the TNBS model of rat colitis: Comparison with ethyl pyruvate." in: Biochemical pharmacology, Vol. 103, pp. 53-63, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Makar, Nimmagadda, Singh, Lam, Mubariz, Judge, Trisler, Bever: "TrkB agonist, 7,8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis." in: Journal of neuroimmunology, Vol. 292, pp. 9-20, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kim, Kim, Wu, Park, Ha, Kim, Lee, Lee, Kwon: "Extracellular Release of CD11b by TLR9 Stimulation in Macrophages." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150677, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Xu, Meng, Erben, Wang, Glauben, Kühl, Wu, Ma, Hu, Wang, Sun, Jia, Wu, Chen, Siegmund, Qin: "Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner." in: Cellular & molecular immunology, 2016

Yang, Scott, Monickaraj, Xu, Ardekani, Nitta, Cabrera, McGuire, Mohideen, Das, Ghosh: "Basement membrane stiffening promotes retinal endothelial activation associated with diabetes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 2, pp. 601-11, 2016

Ulasov, Borovjagin, Kaverina, Schroeder, Shah, Lin, Baryshnikov, Cobbs: "MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo." in: Cancer letters, Vol. 365, Issue 2, pp. 240-50, 2015

Stiess, Wegehingel, Nguyen, Nickel, Bradke, Cambridge: "A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell-Cell Induced Protein Secretion." in: Journal of proteome research, Vol. 14, Issue 8, pp. 3229-38, 2015 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Makar, Gerzanich, Nimmagadda, Jain, Lam, Mubariz, Trisler, Ivanova, Woo, Kwon, Bryan, Bever, Simard: "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 12, pp. 210, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sakaguchi, Shono, Suzuki, Sawano, Anderson, Do, Ohtsubo, Mizunoya, Sato, Nakamura, Furuse, Yamada, Ikeuchi, Tatsumi: "Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle." in: The international journal of biochemistry & cell biology, Vol. 54, pp. 272-85, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Sawano, Suzuki, Do, Ohtsubo, Mizunoya, Ikeuchi, Tatsumi: "Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, Issue 12, pp. 994-1000, 2014

Gil-Pisa, Cebrián, Ortega, Meana, Sulzer: "Cytokine pathway disruption in a mouse model of schizophrenia induced by Munc18-1a overexpression in the brain." in: Journal of neuroinflammation, Vol. 11, pp. 128, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Shono, Sakaguchi, Suzuki, Do, Mizunoya, Nakamura, Sato, Furuse, Yamada, Ikeuchi, Tatsumi: "Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 84, Issue 11, pp. 744-50, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Bray, Reyes, Roberts, Ransohoff, Gruol: "Synaptic plasticity in the hippocampus shows resistance to acute ethanol exposure in transgenic mice with astrocyte-targeted enhanced CCL2 expression." in: Neuropharmacology, Vol. 67, pp. 115-25, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Wong, Zhang, Gilkes, Chen, Wei, Chaturvedi, Hubbi, Semenza: "Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis." in: Journal of molecular medicine (Berlin, Germany), Vol. 90, Issue 7, pp. 803-15, 2012 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Jin, Li, Xu, Guo, Chen, Bo: "Lipoxin A4 analog attenuates morphine antinociceptive tolerance, withdrawal-induced hyperalgesia, and glial reaction and cytokine expression in the spinal cord of rat." in: Neuroscience, Vol. 208, pp. 1-10, 2012 (Sample species: Rat (Rattus)). Further details: Western Blotting

Zhang, Kim: "Effect of molecular size and modification pattern on the internalization of water soluble β-(1 → 3)-(1 → 4)-glucan by primary murine macrophages." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 914-27, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Nelson, Olde Engberink, Hernandez, Puro, Huitron-Resendiz, Hao, De Graan, Gruol: "Altered synaptic transmission in the hippocampus of transgenic mice with enhanced central nervous systems expression of interleukin-6." in: Brain, behavior, and immunity, Vol. 26, Issue 6, pp. 959-71, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Nelson, Hao, Manos, Ransohoff, Gruol: "Altered hippocampal synaptic transmission in transgenic mice with astrocyte-targeted enhanced CCL2 expression." in: Brain, behavior, and immunity, Vol. 25 Suppl 1, pp. S106-19, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yin, Wu, Avigan, Rosenblatt, Stone, Kharbanda, Kufe: "MUC1-C oncoprotein suppresses reactive oxygen species-induced terminal differentiation of acute myelogenous leukemia cells." in: Blood, Vol. 117, Issue 18, pp. 4863-70, 2011

Gruol, Puro, Hao, Blakely, Janneke, Vo: "Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6." in: Journal of neuroimmunology, Vol. 239, Issue 1-2, pp. 28-36, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409930) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409930) anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody
Western Blotting (WB) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409930) Western Blot: Integrin alpha M/CD11b Antibody [ABIN4099304] - Detection of CD11b in R...
Simple Western (SimWes) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409930) Simple Western: Integrin alpha M/CD11b Antibody [ABIN4099304] - Simple Western lane v...
Immunofluorescence (IF) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...
Immunofluorescence (IF) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...