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|Antigen||CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) Antibodies|
|Epitope||AA 99-117, Middle Region Alternatives|
|Conjugate||This CRADD antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-CRADD AntibodyTarget Details CRADD Application Details Handling Images
|Specificity||This antibody detects RAIDD at Center.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human|
|Purification||Peptide Affinity Chromatography|
|Immunogen||RAIDD antibody was raised against a peptide corresponding to amino acids 99 to 117 of human RAIDD.|
Target Details CRADDProduct Details anti-CRADD Antibody Application Details Handling Images back to top
|Alternative Name||RAIDD (CRADD Antibody Abstract)|
|Background||Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1,RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1,3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.Synonyms: CRADD, Caspase and RIP adapter with death domain, Death domain-containing protein CRADD, RIP-associated protein with a death domain|
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-CRADD Antibody Target Details CRADD Handling Images back to top
ELISA. Western blot: 1: 500 - 1: 1000, a 22 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CRADD Antibody Target Details CRADD Application Details Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|