anti-Human GZMA antibody for Immunohistochemistry (Frozen Sections)

Recommended GZMA Antibody (supplied by: Log in to see )

Antigen
Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) Antibodies
  • gzmA
  • GZMA
  • AW494114
  • Ctla-3
  • Ctla3
  • Hf
  • SE1
  • TSP-1
  • TSP1
  • CTLA3
  • HFSP
  • granzyme A
  • Granzyme A
  • granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
  • gzmA
  • GZMA
  • graa
  • Gzma
Epitope
AA 29-262
22
7
4
4
3
3
3
2
2
2
1
1
1
1
Reactivity
Human
122
31
22
3
2
1
1
1
1
1
1
Host
Rabbit
63
56
13
Clonality
Polyclonal
Conjugate
This GZMA antibody is un-conjugated
6
5
5
5
5
4
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
49
44
35
23
19
13
7
6
4
3
3
2
1
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Catalog No. ABIN1859107
$ 314.00
Plus shipping costs $45.00

General

Antigen Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) Antibodies
Epitope AA 29-262
(22), (7), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(122), (31), (22), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(63), (56), (13)
Clonality
Polyclonal
Conjugate This GZMA antibody is un-conjugated
(6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(49), (44), (35), (23), (19), (13), (7), (6), (4), (3), (3), (2), (1)
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Product Details anti-GZMA Antibody

Target Details GZMA Application Details Handling Images
Sequence YVEFHHHHHH -II GGNEVTPHSR PYMVLLSLDR KTICAGALIA KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE FPYPCYDPAT REGDLKLLQL MEKAKINKYV TILHLPKKGD DVKPGTMCQV AGWGRTHNSA SWSDTLREVN ITIIDRKVCN DRNHYNFNPV IGMNMVCAGS LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP GVYILLSKKH LNWIIMTIKG AV
Specificity It has been selected for its ability to recognize GZMA in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant GZMA expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against GZMA.
Isotype IgG

Target Details GZMA

Product Details anti-GZMA Antibody Application Details Handling Images back to top
Antigen
Alternative Name Granzyme A (GZMA Antibody Abstract)
Research Area Cell/Tissue Markers
Pathways Apoptosis

Application Details

Product Details anti-GZMA Antibody Target Details GZMA Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant GZMA (Ile29~Val262) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

Product Details anti-GZMA Antibody Target Details GZMA Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-GZMA Antibody Target Details GZMA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-262) antibody (ABIN1859107) anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-262) antibody