GZMA antibody (Middle Region)

Catalog No. ABIN3044452

Product Details anti-GZMA Antibody

Target: GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))

Binding Specificity: AA 175-190, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Granzyme A(GZMA) detection. Tested with WB, IHC-P in Human.

Sequence: DRKVCNDRNH YNFNPV

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Granzyme A(GZMA) detection. Tested with WB, IHC-P in Human.
Gene Name: granzyme A(granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
Protein Name: Granzyme A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Granzyme A(175-190aa DRKVCNDRNHYNFNPV).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GZMA

Target: GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))

Alternative Name: GZMA (GZMA Products)

Synonyms: gzmA antibody, GZMA antibody, AW494114 antibody, Ctla-3 antibody, Ctla3 antibody, Hf antibody, SE1 antibody, TSP-1 antibody, TSP1 antibody, CTLA3 antibody, HFSP antibody, granzyme A antibody, Granzyme A antibody, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) antibody, gzmA antibody, GZMA antibody, graa antibody, Gzma antibody

Background: Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes(CTL) and natural killer(NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are though to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A .

Synonyms: CTL tryptase antibody|CTLA3 antibody|Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody|Cytotoxic T lymphocyte associated serine esterase 3 antibody|Cytotoxic T lymphocyte proteinase 1 antibody|Cytotoxic T-lymphocyte proteinase 1 antibody|Fragmentin 1 antibody|Fragmentin-1 antibody|GRAA_HUMAN antibody|Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody|Granzyme A(Cytotoxic T lymphocyte associated serine esterase 3, Hanukah factor serine protease) antibody|Granzyme A(granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody|Granzyme A antibody|Granzyme A precursor antibody|Granzyme-1 antibody|GZMA antibody|H factor antibody|Hanukah factor serine protease antibody|Hanukkah factor antibody|HF antibody|HFSP antibody|TSP1 antibody

UniProt: P12544

Pathways: Apoptosis