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anti-Human JAK3 antibody for Immunohistochemistry (Frozen Sections)

Recommended JAK3 Antibody (supplied by: Log in to see )

Antigen
Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
  • fae
  • JAK
  • JAK-3
  • JAK3_HUMAN
  • JAKL
  • L-JAK
  • LJAK
  • RATJAK3
  • wil
Epitope
AA 716-967
15
15
13
13
11
8
7
7
5
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
83
58
41
2
2
2
2
1
1
1
Host
Rabbit
63
23
1
Clonality
Polyclonal
Conjugate
This JAK3 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
50
37
23
15
12
10
8
6
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN1859526
$ 385.00
Plus shipping costs $45.00

Similar anti-JAK3 Antibodies

Application / Reactivity Human
ELISA 37 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
Epitope AA 716-967
(15), (15), (13), (13), (11), (8), (7), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(83), (58), (41), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(63), (23), (1)
Clonality
Polyclonal
Conjugate This JAK3 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(50), (37), (23), (15), (12), (10), (8), (6), (1), (1), (1), (1), (1)
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Product Details anti-JAK3 Antibody

Target Details JAK3 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-WEVFS GVTMPISALD PAKKLQFYED RQQLPAPKWT ELALLIQQCM AYEPVQRPSF RAVIRDLNSL ISSDYELLSD PTPGALAPRD GLWNGAQLYA CQDPTIFEER HLKYISQLGK GNFGSVELCR YDPLGDNTGA LVAVKQLQHS GPDQQRDFQR EIQILKALHS DFIVKYRGVS YGPGRQSLRL VMEYLPSGCL RDFLQRHRAR LDASRLLLYS SQICKGMEYL GSRRCVHRDL AARNILVESE AHVKIAD
Specificity It has been selected for its ability to recognize JAK3 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen JAK3 (AA 716-967)
Isotype IgG

Target Details JAK3

Product Details anti-JAK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Janus Kinase 3 (JAK3) (JAK3 Antibody Abstract)
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-JAK3 Antibody Target Details JAK3 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant JAK3 (Trp716~Asp967) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-JAK3 antibody (Tyrosine-Protein Kinase JAK3) (AA 716-967) (ABIN1859526) anti-Tyrosine-Protein Kinase JAK3 (JAK3) (AA 716-967) antibody