JAK3 antibody (AA 720-800)

Catalog No. ABIN6284966

Product Details anti-JAK3 Antibody

Target: JAK3 (Janus Kinase 3 (JAK3))

Binding Specificity: AA 720-800

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: JAK3 is a protein encoded by the JAK3 gene which is approximately 125 kDa. JAK3 is localised to the endomembrane system. It is involved in RET signalling, common cytokine receptor gamma-chain family signalling pathways, Th17 cell differentiation and the IL-2 pathway. It is a non-receptor tyrosine kinase involved in various processes such as cell growth, development and differentiation. It mediates essential signalling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm it also plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R and IL7R. JAK3 is only expressed in NK cells and NK-like cell lines. Mutations in the JAK3 gene may result in severe combined immunodeficiency and Janus kinase-3 deficiency. STJ93795 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of JAK3 protein.

Specificity: JAK3 Polyclonal Antibody detects endogenous levels of JAK3 protein.

Characteristics: Rabbit polyclonal to JAK3.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JAK3 around the non-phosphorylation site of Y785.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Comment:

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Alternative Name: JAK3 (JAK3 Products)

Synonyms: JAK-3 antibody, JAK3_HUMAN antibody, JAKL antibody, L-JAK antibody, LJAK antibody, fae antibody, wil antibody, RATJAK3 antibody, JAK antibody, Janus kinase 3 antibody, JAK3 antibody, Jak3 antibody

Molecular Weight: 125 kDa

Gene ID: 3718

UniProt: P52333

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response