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anti-Rat (Rattus) PIAS1 antibody for Immunocytochemistry

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Epitope
AA 636-651
33
28
18
5
3
3
3
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
87
36
19
5
4
3
3
3
3
2
2
2
1
1
Host
Rabbit
82
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
85
56
51
18
8
6
4
3
3
2
1
1
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Available images

Catalog No. ABIN3032369
$ 391.88
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3044474 ICC IHC (p) WB Rabbit IgG AA 636-651, C-Term Log in to see Polyclonal
1 ABIN4345417 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2626785 ICC IHC (p) WB Rabbit IgG AA 636-651 Log in to see Polyclonal

Similar anti-PIAS1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 9 Antibodies 14 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 13 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Western Blotting (WB) 20 Antibodies 36 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope AA 636-651
(33), (28), (18), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(87), (36), (19), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(82), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(85), (56), (51), (18), (8), (6), (4), (3), (3), (2), (1), (1)
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling Images
Purification Antigen affinity
Immunogen Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat).
Isotype IgG

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
Application Notes The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
Buffer 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage -20 °C
Storage Comment After reconstitution, the PIAS1 antibody can be stored for up to one month at 4 °C. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3032369) ICC testing of PIAS1 antibody and A549 cells
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3032369) IHC-P: PIAS1 antibody testing of human placenta tissue
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3032369) IHC-P: PIAS1 antibody testing of rat brain tissue
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3032369) Western blot testing of PIAS1 antibody and Lane 1: rat testis; 2: mouse testis; 3:...
 image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3032369) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651) antibody (Image 5)