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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||8 Antibodies||13 Antibodies||52 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies||7 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||3 Antibodies|
|Western Blotting (WB)||17 Antibodies||31 Antibodies|
|Antigen||Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies|
|Epitope||AA 636-651, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PIAS1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PIAS1 AntibodyTarget Details PIAS1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: protein inhibitor of activated STAT, 1
Protein Name: E3 SUMO-protein ligase PIAS1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1(636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.|
Target Details PIAS1Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
|Alternative Name||PIAS1 (PIAS1 Antibody Abstract)|
|Background||E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors|
|Pathways||JAK-STAT Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling back to top