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anti-Human MAP2K5 antibody for Enzyme Immunoassay

Recommended MAP2K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
  • AI324775
  • AI428457
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • fb73f02
  • HsT17454
  • MAP2K_A
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAPKK5
  • Mapkk5
  • Mek5
  • MEK5
  • PRKMK5
  • Prkmk5
  • wu:fb73f02
  • zgc:172137
Epitope
Ser149
32
31
17
16
15
13
13
13
13
6
5
5
4
4
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
153
76
76
11
9
7
6
5
3
3
3
3
2
2
1
Host
Rabbit
155
16
Clonality
Polyclonal
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
5
5
5
5
5
5
5
5
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
54
47
29
22
21
4
2
2
1
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Catalog No. ABIN360284
$ 390.50
Plus shipping costs $45.00

Top referenced anti-MAP2K5 antibody for Enzyme Immunoassay

Similar anti-MAP2K5 Antibodies

Application / Reactivity Human
ELISA 46 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 54 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 96 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
Epitope Ser149
(32), (31), (17), (16), (15), (13), (13), (13), (13), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(153), (76), (76), (11), (9), (7), (6), (5), (3), (3), (3), (3), (2), (2), (1)
Host Rabbit
(155), (16)
Clonality
Polyclonal
Conjugate This MAP2K5 antibody is un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(111), (54), (47), (29), (22), (21), (4), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-MAP2K5 Antibody

Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) Images
Specificity This antibody reacts to MAP2K5.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S149 of human MAP2K5.
Isotype Ig

Target Details MAP2K5

Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
Antigen
Alternative Name MAP2K5 (MAP2K5 Antibody Abstract)
Background MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5
Gene ID 5607, 9606
UniProt Q13163
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP2K5 Antibody (ABIN360284)

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Background publications

Raviv, Kalie, Seger: "MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation." in: Journal of cell science, Vol. 117, Issue Pt 9, pp. 1773-84, 2004

English, Pearson, Hockenberry, Shivakumar, White, Cobb: "Contribution of the ERK5/MEK5 pathway to Ras/Raf signaling and growth control." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31588-92, 1999

Kato, Kravchenko, Tapping, Han, Ulevitch, Lee: "BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C." in: The EMBO journal, Vol. 16, Issue 23, pp. 7054-66, 1998

Images

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) back to top
Supplier Images
 image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (Ser149) (ABIN360284) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (Ser149) antibody
 image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (Ser149) (ABIN360284) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (Ser149) antibody (Image 2)