|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||21 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||54 Antibodies|
|Immunohistochemistry (IHC)||24 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||91 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies|
|Conjugate||This MAP2K5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MAP2K5 AntibodyTarget Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) Images
|Specificity||This antibody reacts to MAP2K5.|
|Purification||Protein A column, followed by peptide affinity purification|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S149 of human MAP2K5.|
Target Details MAP2K5Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
|Alternative Name||MAP2K5 (MAP2K5 Antibody Abstract)|
|Background||MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5|
|Gene ID||5607, 9606|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN360284) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP2K5 Antibody (ABIN360284)Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Raviv, Kalie, Seger: "MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation." in: Journal of cell science, Vol. 117, Issue Pt 9, pp. 1773-84, 2004 (PubMed).
English, Pearson, Hockenberry et al.: "Contribution of the ERK5/MEK5 pathway to Ras/Raf signaling and growth control." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31588-92, 1999 (PubMed).
Kato, Kravchenko, Tapping et al.: "BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C." in: The EMBO journal, Vol. 16, Issue 23, pp. 7054-66, 1998 (PubMed).
ImagesProduct Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN360284) back to top