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anti-Human MAP2K5 antibody for Western Blotting

Recommended MAP2K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
  • AI324775
  • AI428457
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • fb73f02
  • HsT17454
  • MAP2K_A
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAPKK5
  • Mapkk5
  • Mek5
  • MEK5
  • PRKMK5
  • Prkmk5
  • wu:fb73f02
  • zgc:172137
Epitope
AA 380-430
31
30
17
17
15
13
13
13
12
6
5
5
4
4
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
75
74
11
9
7
6
5
3
3
3
3
2
2
1
Host
Rabbit
154
16
Clonality
Polyclonal
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
5
5
5
5
5
5
5
5
4
4
4
4
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
110
53
47
29
22
21
4
2
2
1
1
Supplier
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Available images

Catalog No. ABIN754183
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN392401 IHC (p) WB Rabbit Ig AA 127-156, Ser149 Log in to see Polyclonal 1
0.13476625 ABIN393416 IF WB Mouse IgG1 kappa Met11 Log in to see 3E10 5
0.13476625 ABIN968273 IF WB Mouse IgG1 AA 13-188 Log in to see 21-MEK5 2
0.13476625 ABIN968272 IF WB Mouse IgG1 AA 13-188 Log in to see LPR-01 2
0.13476625 ABIN126841 WB Mouse IgG1 Log in to see 14B5
0.13476625 ABIN658075 WB Rabbit Ig AA 385-412, C-Term Log in to see Polyclonal
0.13476625 ABIN2783608 WB Rabbit N-Term Log in to see Polyclonal
0.13476625 ABIN189531 ICC IF WB Rabbit IgG Log in to see EP648Y
0.13476625 ABIN1870358 WB Rabbit IgG pSer311, pThr315 Log in to see Polyclonal
0.13476625 ABIN519261 IF WB Mouse AA 1-448, full length Log in to see Polyclonal
0.13476625 ABIN2441139 WB Rabbit pSer311, pThr315 Log in to see Polyclonal
0.13476625 ABIN928258 WB Rabbit N-Term Log in to see Polyclonal
0.13476625 ABIN562429 IF ELISA WB Mouse IgG1 kappa AA 1-448, full length Log in to see M1-E6
0.13476625 ABIN562434 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 3E10
0.13476625 ABIN2447941 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13476625 ABIN2445307 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.13476625 ABIN3181576 IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN3020325 WB Rabbit pSer311, pThr315 Log in to see Polyclonal
0.0011760078 ABIN360284 EIA IHC (p) WB Rabbit Ig Ser149 Log in to see Polyclonal 3
0.0011760078 ABIN372667 WB Rabbit IgG pSer311, pThr315 Log in to see Polyclonal 1

Top referenced anti-MAP2K5 antibody for Western Blotting

Similar anti-MAP2K5 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 6 Antibodies 6 Antibodies 46 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 5 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 32 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 45 Antibodies 43 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
Epitope AA 380-430
(31), (30), (17), (17), (15), (13), (13), (13), (12), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(153), (75), (74), (11), (9), (7), (6), (5), (3), (3), (3), (3), (2), (2), (1)
Host Rabbit
(154), (16)
Clonality
Polyclonal
Conjugate This MAP2K5 antibody is un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(110), (53), (47), (29), (22), (21), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP2K5 Antibody

Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN754183) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MEK5
Isotype IgG

Target Details MAP2K5

Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN754183) Images back to top
Antigen
Alternative Name Mek5 (MAP2K5 Antibody Abstract)
Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Subcellular location: Cytoplasm, Nucleus


Synonyms: ASK1, MEKK5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, MAP3K5
Gene ID 4217
UniProt Q99683
Research Area Cancer, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN754183) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN754183) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-MAP2K5 Antibody (ABIN754183)

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Product cited in:

Chen, Hou, Ye, Chen, Cui, Tian, Li, Liu: "MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling." in: Scientific reports, Vol. 4, pp. 3819, 2014

Li, Dong, Wei, Wang, Zhang, Li: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, 2014

Images

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN754183) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 P...
Immunohistochemistry (IHC) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 fo...
'Independent Validation' Badge
Lot Number 120828
Method validated Immunohistochemistry
Positive Control Human breast myoepithelial and glandular tissue
Negative Control Human breast adipose tissue
Notes Strong signal was detected in positive control sample, with minor background seen in negative control sample.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)
  • Catalog number: ABIN754183
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 120828
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
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  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human breast myoepithelial and glandular tissue stained with antibody
  • Negative control: Human breast adipose tissue stained with antibody
  • Isotype control: Human breast myoepithelial and glandular tissue stained with isotype control
  • Secondary only control: Human breast myoepithelial and glandular tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 1: Human breast tissue stained with anti-MAP2K5 (brown) and counterstained wit...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 2: Human breast adipose tissue stained with anti-MAP2K5 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 3: Human breast tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 4: Human breast tissue stained with secondary antibody only (brown) and counte...