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anti-Mouse (Murine) MAP2K7 antibody for Immunocytochemistry

Recommended MAP2K7 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies
  • 2
  • 5930412N11Rik
  • JNKK
  • JNKK 2
  • Jnkk2
  • MAP2K7
  • map2k7-A
  • MAPKK 7
  • MAPKK7
  • Mapkk7
  • MEK 7
  • Mek7
  • MKK7
  • Mkk7
  • Prkmk7
  • PRKMK7
  • SAPKK-4
  • SAPKK4
  • sek2
  • si:ch211-254d18.2
Epitope
C-Term
29
27
20
15
13
9
7
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
120
61
51
1
1
1
Host
Rabbit
107
21
Clonality
Monoclonal
Conjugate
This MAP2K7 antibody is un-conjugated
5
5
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
109
68
42
15
12
11
8
5
5
3
2
1
1
Supplier
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Available images

Catalog No. ABIN189534
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN1873610 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN1859747 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 181-429 Log in to see Polyclonal
0.0010161072 ABIN2405246 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN2973668 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-MAP2K7 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 25 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 8 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies
Epitope C-Term
(29), (27), (20), (15), (13), (9), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(120), (61), (51), (1), (1), (1)
Host Rabbit
(107), (21)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K7 antibody is un-conjugated
(5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(109), (68), (42), (15), (12), (11), (8), (5), (5), (3), (2), (1), (1)
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Product Details anti-MAP2K7 Antibody

Target Details MAP2K7 Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues on the C-terminus of human MKK7 was used as an immunogen.
Clone EP1455Y
Isotype IgG

Target Details MAP2K7

Product Details anti-MAP2K7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K7 (MAP2K7 Antibody Abstract)
Background C-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activatedprotein (MAP) kinase family, regulates gene expression in response to variousextracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, wasphosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNKsignaling cascade. MKK7 activity was also stimulated by constitutively active forms ofRac and Cdc42Hs, members of the Rho small GTP-binding protein family (1).endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (butnot interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor forthe Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposedto heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokinetumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiologicalpathways (2). Alternate Names: anti-c-Jun N-terminal kinase kinase 2 antibody, anti-Dual specificity mitogen activatedprotein kinase kinase 7 antibody, anti-JNK activating kinase 2 antibody, anti-JNK kinase2 antibody, anti-Jnkk 2 antibody, anti-Jnkk-2 antibody, anti-Jnkk2 antibody, anti-MAPkinase kinase 7 antibody, anti-MAP2K7 antibody, anti-MAPKK 7 antibody,anti-MAPKK-7 antibody, anti-MAPKK7 antibody, anti-Mitogen Activated Protein Kinasekinase 7 antibody, anti-MKK 7 antibody, anti-MKK-7 antibody, anti-MKK7 antibody,anti-PRKMK 7 antibody, anti-PRKMK-7 antibody, anti-PRKMK7 antibody, anti-Sek 2antibody, anti-Sek-2 antibody, anti-Sek2 antibody.
Gene Symbol: MAP2K7
Gene ID 5609, 26400, 363855
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 47kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:100, Western Blot 1:500
Protocol Protocol specific for MEK7 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K7 antibody (Mitogen-Activated Protein Kinase Kinase 7) (C-Term) (ABIN189534) Western blot analysis on HeLa cell lysate using anti-MKK7 RabMAb (cat. # 110-57209), ...
Immunohistochemistry (IHC) image for anti-MAP2K7 antibody (Mitogen-Activated Protein Kinase Kinase 7) (C-Term) (ABIN189534) Immunohistochemical staining of paraffin-embedded human urinary bladder using anti-MK...