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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||21 Antibodies||25 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies||20 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies||8 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||42 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K7 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-MAP2K7 AntibodyTarget Details MAP2K7 Application Details Handling Images
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues on the C-terminus of human MKK7 was used as an immunogen.|
Target Details MAP2K7Product Details anti-MAP2K7 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K7 (MAP2K7 Antibody Abstract)|
C-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activatedprotein (MAP) kinase family, regulates gene expression in response to variousextracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, wasphosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNKsignaling cascade. MKK7 activity was also stimulated by constitutively active forms ofRac and Cdc42Hs, members of the Rho small GTP-binding protein family (1).endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (butnot interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor forthe Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposedto heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokinetumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiologicalpathways (2). Alternate Names: anti-c-Jun N-terminal kinase kinase 2 antibody, anti-Dual specificity mitogen activatedprotein kinase kinase 7 antibody, anti-JNK activating kinase 2 antibody, anti-JNK kinase2 antibody, anti-Jnkk 2 antibody, anti-Jnkk-2 antibody, anti-Jnkk2 antibody, anti-MAPkinase kinase 7 antibody, anti-MAP2K7 antibody, anti-MAPKK 7 antibody,anti-MAPKK-7 antibody, anti-MAPKK7 antibody, anti-Mitogen Activated Protein Kinasekinase 7 antibody, anti-MKK 7 antibody, anti-MKK-7 antibody, anti-MKK7 antibody,anti-PRKMK 7 antibody, anti-PRKMK-7 antibody, anti-PRKMK7 antibody, anti-Sek 2antibody, anti-Sek-2 antibody, anti-Sek2 antibody.
Gene Symbol: MAP2K7
|Gene ID||5609, 26400, 363855|
|Pathways||MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 47kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:100, Western Blot 1:500
Protocol specific for MEK7 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Handling back to top