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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||4 Antibodies||29 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies||25 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||4 Antibodies|
|Western Blotting (WB)||10 Antibodies||42 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAP3K12 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K12 AntibodyTarget Details MAP3K12 Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) Images
|Specificity||This antibody detects MAP3K12 at C-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ZPK.|
Target Details MAP3K12Product Details anti-MAP3K12 Antibody Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
|Alternative Name||MAP3K12 (MAP3K12 Antibody Abstract)|
|Background||ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.Synonyms: Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase|
|Molecular Weight||93188 Da|
|Gene ID||7786, 9606|
Application DetailsProduct Details anti-MAP3K12 Antibody Target Details MAP3K12 Handling References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-MAP3K12 Antibody (ABIN359408)Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling Images back to top
Itoh, Wang, Ito et al.: "SP3 acts as a positive regulator on the core promoter of human ZPK gene." in: Biochemical and biophysical research communications, Vol. 313, Issue 3, pp. 612-8, 2003 (PubMed).
Fukuyama, Yoshida, Yamashita et al.: "MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21247-54, 2000 (PubMed).
Reddy, Basu, Bannerman et al.: "ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation." in: Oncogene, Vol. 18, Issue 31, pp. 4474-84, 1999 (PubMed).
Curran: "It is better to travel hopefully ellipsis" in: Chemistry & biology, Vol. 3, Issue 8, pp. 641-2, 1996 (PubMed).
Mata, Merritt, Fan et al.: "Characterization of dual leucine zipper-bearing kinase, a mixed lineage kinase present in synaptic terminals whose phosphorylation state is regulated by membrane depolarization via calcineurin." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16888-96, 1996 (PubMed).
ImagesProduct Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) back to top