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|Application / Reactivity||Human||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Western Blotting (WB)||52 Antibodies||11 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunohistochemistry (IHC)||20 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 2 (MAP3K2) Antibodies|
|Epitope||AA 606-619, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This MAP3K2 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-MAP3K2 AntibodyTarget Details MAP3K2 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 2(MAP3K2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase kinase 2
Protein Name: Mitogen-activated protein kinase kinase kinase 2
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK2(606-619aa SADELLRHMFVHYH), different from the related rat and mouse sequences by one amino acid.|
Target Details MAP3K2Product Details anti-MAP3K2 Antibody Application Details Handling Images back to top
|Alternative Name||MAP3K2 (MAP3K2 Antibody Abstract)|
|Background||MAP3K2, mitogen-activated protein kinase kinase kinase 2, also known as MEKK2, belongs to the mitogen-activated protein kinase(MAPK) kinase kinase gene family, which is involved in regulating multiple MAPK signaling pathways. Endogenous MAP3K2 localized mainly to the cytosol of unstimulated HeLa cells and rat fibroblasts. The MAP3K2 gene is mapped to chromosome 2 by the International Radiation Hybrid Mapping Consortium. MAP3K2 is a major upstream activator of the JNK, MAPK cascade. MAP3K2 was identifies as 1 of 6 kinases that regulate different steps of the caveolar cycle. Silencing of MAP3K2 strongly reduced kiss-and-run dynamics, leading to an accumulation of caveolar structures at the cell surface without affecting caveolae coat assembly or clustering.|
Application DetailsProduct Details anti-MAP3K2 Antibody Target Details MAP3K2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K2 Antibody Target Details MAP3K2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-MAP3K2 Antibody Target Details MAP3K2 Application Details Handling back to top