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anti-Cat (Feline) MAP1B antibody for Immunohistochemistry (Frozen Sections)

Recommended MAP1B Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 1B (MAP1B) Antibodies
  • A230055D22
  • AI843217
  • FUTSCH
  • LC1
  • MAP5
  • Mtap-5
  • Mtap1b
  • Mtap5
Reactivity
Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
53
45
41
17
17
15
11
11
Host
Mouse
46
18
Clonality
Monoclonal
Conjugate
This MAP1B antibody is un-conjugated
5
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
31
27
23
18
15
11
10
6
3
1
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Catalog No. ABIN269612
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Similar anti-MAP1B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 5 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 10 Antibodies
Immunofluorescence (IF) 18 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Microarray (MA) 1 Antibodies
Western Blotting (WB) 26 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen Microtubule-Associated Protein 1B (MAP1B) Antibodies
Reactivity Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
(53), (45), (41), (17), (17), (15), (11), (11)
Host Mouse
(46), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP1B antibody is un-conjugated
(5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
(31), (27), (23), (18), (15), (11), (10), (6), (3), (1)
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Product Details anti-MAP1B Antibody

Target Details MAP1B Application Details Handling Images
Specificity The antibody does not cross-react with other MAPs or tubulin.
Cross-Reactivity (Details) May react with Hamster.
Purification Ascites
Immunogen Rat brain microtubule associated proteins.
Clone AA6
Isotype IgG1

Target Details MAP1B

Product Details anti-MAP1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP1B (MAP1B Antibody Abstract)
Background Alternate Names: Anti-DKFZp686E1099 antibody, Anti-DKFZp686F1345 antibody, Anti-FLJ38954antibody, Anti-FUTSCH antibody, Anti-MAP 1B antibody, Anti-MAP5 antibody,Anti-Microtubule-associated protein 1B antibody.
Gene Symbol: MAP1B
Gene ID 4131, 17755, 29456
Research Area Cytoskeleton
Pathways Microtubule Dynamics

Application Details

Product Details anti-MAP1B Antibody Target Details MAP1B Handling Images back to top
Application Notes Recommended dilutions: Microarray, Western Blot 1:500, Immunohistochemistry-Frozen 1:10-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:10-1:2000
Protocol Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
Restrictions For Research Use only

Handling

Product Details anti-MAP1B Antibody Target Details MAP1B Application Details Images back to top
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.