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anti-Human ROCK1 antibody for ELISA

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • 1110055K06Rik
  • CG9774
  • Dmel\\CG9774
  • DRhk
  • dRok
  • drok
  • Drok
  • DROK
  • DRok
  • dROK
  • LOC100229794
  • P160ROCK
  • P160Rock
  • p160ROCK
  • Rhk
  • RhoK
  • ROCK
  • Rock
  • ROCK-1
  • ROCK-I
  • Rock-I
  • ROCK1
  • ROK
  • Rok
  • ROKalpha/beta
Reactivity
Human
142
98
94
21
19
19
3
3
2
2
1
Host
Mouse
109
38
Clonality
Monoclonal
Conjugate
This ROCK1 antibody is un-conjugated
4
4
4
4
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
108
32
30
22
17
17
10
8
2
1
1
1
1
Supplier
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Catalog No. ABIN969563
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32693866 ABIN562671 IF ELISA WB Mouse IgG1 kappa AA 401-510, partial Log in to see 2E2 2
0.32693866 ABIN1846125 ELISA WB Mouse IgG1 Log in to see
0.0012117105 ABIN1500698 ELISA WB Mouse IgG1 Log in to see 1H4
0.0012117105 ABIN948412 ELISA WB Mouse IgG1 kappa AA 401-510, partial Log in to see 2E2
0.0012117105 ABIN2499974 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN1585151 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN1453860 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012117105 ABIN3186829 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012117105 ABIN3186828 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012117105 ABIN1828788 IF ELISA WB Mouse IgG1, kappa Log in to see 2E2
0.0012117105 ABIN1091629 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1091630 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1887940 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2353153 ELISA WB Mouse IgG1 Log in to see 13B679
0.0012117105 ABIN2499975 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2428675 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2422119 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2885883 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal
0.0012117105 ABIN1585150 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-ROCK1 antibody for ELISA

Similar anti-ROCK1 Antibodies

Application / Reactivity Human
ELISA 29 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Reactivity Human
(142), (98), (94), (21), (19), (19), (3), (3), (2), (2), (1)
Host Mouse
(109), (38)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(108), (32), (30), (22), (17), (17), (10), (8), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN969563) Images
Purification Purified
Immunogen Purified recombinant fragment of human ROCK1 expressed in E. coli.
Clone 1H4
Isotype IgG1

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 Antibody (ABIN969563) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Molecular Weight 158 kDa
Gene ID 6093
HGNC 6093
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 Antibody (ABIN969563) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 Antibody (ABIN969563) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-ROCK1 Antibody (ABIN969563)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Product cited in:

Kroll, Epting, Kern, Dietz, Feng, Hammes, Wieland, Augustin: "Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H893-9, 2009

Narumiya, Tanji, Ishizaki: "Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion." in: Cancer metastasis reviews, Vol. 28, Issue 1-2, pp. 65-76, 2009

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN969563) back to top
Supplier Images
ELISA image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (ABIN969563) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (ABIN969563) Figure 2: Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) rec...
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (ABIN969563) Figure 3: Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403...