anti-Human ROCK1 antibody for Immunofluorescence (Cultured Cells)

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • ROCK1
  • LOC100229794
  • rok
  • Rock1
  • rock1
Epitope
AA 107-371, N-Term
38
28
27
24
21
15
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
195
144
133
25
21
20
6
5
4
2
1
Host
Rabbit
114
83
Clonality
Polyclonal
Conjugate
This ROCK1 antibody is un-conjugated
8
7
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
154
45
36
33
28
24
23
15
2
2
1
1
Options
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Catalog No. ABIN2800511
$ 431.20
Plus shipping costs $45.00

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope AA 107-371, N-Term
(38), (28), (27), (24), (21), (15), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(195), (144), (133), (25), (21), (20), (6), (5), (4), (2), (1)
Host Rabbit
(114), (83)
Clonality
Polyclonal
Conjugate This ROCK1 antibody is un-conjugated
(8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(154), (45), (36), (33), (28), (24), (23), (15), (2), (2), (1), (1)
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling Images
Immunogen Recombinant protein fragment contain a sequence corresponding to a region within amino acids 107-371 (Q13464) of ROCK1
Isotype IgG

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background Synonyms: MGC131603 antibody, MGC43611 antibody, P160ROCK antibody, PRO0435 antibody, ROCK1 antibody, p160 ROCK-1 antibody, rho-associated, coiled-coil-containing protein kinase 1 antibody, rho-associated protein kinase 1 antibody, renal carcinoma antigen NY-REN-35 antibody, rho-associated, coiled-coil-containing protein kinase I antibody, Rho kinase antibody, p160-ROCK antibody, Rho-associated, coiled-coil containing protein kinase 1 antibody
Molecular Weight 158 kDa
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling Images back to top
Application Notes Tested ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 ?g of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 ?g of ROCK1 antibody (107-11050) 5 % SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (107-11050) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell extract B. 30 ?g C2Cl2 whole cell extract5 % SDS-PAGEROCK1 antibody [N1N2], N-term (107-11050) dilution: 1:1000ROCK1 antibody [N1N2], N-term detects ROCK1 protein by Western blot analysis.A. 50 μg rat brain lysate/extract5 % SDS-PAGEROCK1 antibody [N1N2], N-term (107-11050) dilution: 1:500Immunofluorescence analysis of methanol-fixed HeLa, using ROCK1(107-11050) antibody at 1:200 dilution.Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using ROCK1(107-11050) antibody at 1:500 dilution.Sample (30 μg of whole cell lysate) A: A431 B: H1299 C: Molt-4 5 % SDS PAGE 107-11050 diluted at 1:1000
Comment

ROCK1 antibody [N1N2], N-Terminus, 0.68 mg/ml (100 μl)

Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Images back to top
Format Liquid
Concentration 0.68 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.