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anti-Human ROCK1 antibody for Immunohistochemistry

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • 1110055K06Rik
  • CG9774
  • Dmel\\CG9774
  • DRhk
  • dRok
  • drok
  • Drok
  • DROK
  • DRok
  • dROK
  • LOC100229794
  • P160ROCK
  • P160Rock
  • p160ROCK
  • Rhk
  • RhoK
  • ROCK
  • Rock
  • ROCK-1
  • ROCK-I
  • Rock-I
  • ROCK1
  • ROK
  • Rok
  • ROKalpha/beta
Epitope
C-Term
28
25
24
20
15
13
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
142
97
94
21
19
19
3
3
2
2
1
Host
Rabbit
108
39
Clonality
Monoclonal
Conjugate
This ROCK1 antibody is un-conjugated
4
4
4
4
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
108
32
31
21
16
16
9
7
2
1
1
1
1
Supplier
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Catalog No. ABIN258448
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22919032 ABIN2428675 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN2422119 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN152055 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011344256 ABIN2892918 IHC WB Rabbit Asn311 Log in to see Polyclonal
0.0011344256 ABIN1585151 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011344256 ABIN1453860 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011344256 ABIN3186829 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011344256 ABIN446984 IHC (p) IHC WB Rabbit Asp311 Log in to see Polyclonal
0.0011344256 ABIN1885954 IF IHC WB Rabbit AA 107-381 Log in to see Polyclonal
0.0011344256 ABIN2885883 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal
0.0011344256 ABIN2700753 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN2353151 IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011344256 ABIN2353152 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN2353150 IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011344256 ABIN2800511 IF (cc) IHC (f) IF IP IHC (p) IHC WB Rabbit IgG AA 107-371, N-Term Log in to see Polyclonal
0.0011344256 ABIN4350898 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN4350899 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN2988395 IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-ROCK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 17 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 10 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 32 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope C-Term
(28), (25), (24), (20), (15), (13), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(142), (97), (94), (21), (19), (19), (3), (3), (2), (2), (1)
Host Rabbit
(108), (39)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(108), (32), (31), (21), (16), (16), (9), (7), (2), (1), (1), (1), (1)
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling Images
Specificity This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen.
Clone EP786Y
Isotype IgG

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine proteinkinase and an effector of the small GTPase Rho. With N-terminus protein kinase domainand C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can beactivated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho proteinbinds to the Rho-binding domain and induces a conformational change which opens thekinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIMkinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1,Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitoryc-terminal domain. This activation, independent of Rho activity, leads to apoptoticmembrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly,cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth.Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1an ideal target for drug development (3). Alternate Names: anti-MGC131603 antibody, anti-MGC43611 antibody, anti-NY REN 35 antigen antibody,anti-p160 ROCK1 antibody, anti-p160ROCK antibody, anti-Renal carcinoma antigen NYREN 35 antibody, anti-Rho associated coiled coil containing protein kinase 1 antibody,anti-Rho associated protein kinase 1 antibody.
Gene Symbol: ROCK1
Gene ID 6093, 19877, 81762
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling Images back to top
Application Notes In Western blot this antibody detects a bands at approximately 30 & 160 kDa, corresponding to both C-terminal cleaved fragment and full length ROCK1. Immunohidtochemistry Frozen reported in literature (PMID: 19295659)
Recommended dilutions: Flow Cytometry 1:1000, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:100, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for ROCK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (C-Term) (ABIN258448) Western blot analysis anti-ROCK1 RabMAb on untreated HeLa cell lysate (A) and camptot...
Immunohistochemistry (IHC) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (C-Term) (ABIN258448) Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using...
Immunofluorescence (IF) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (C-Term) (ABIN258448) Immunofluorescent staining of HeLa cells using anti-ROCK1 RabMAb (cat. #110-57465).