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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||17 Antibodies||17 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies||6 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||32 Antibodies||32 Antibodies|
|Immunohistochemistry (IHC)||8 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||60 Antibodies|
|Antigen||rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ROCK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-ROCK1 AntibodyTarget Details ROCK1 Application Details Handling Images
|Specificity||This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen.|
Target Details ROCK1Product Details anti-ROCK1 Antibody Application Details Handling Images back to top
|Alternative Name||ROCK1 (ROCK1 Antibody Abstract)|
Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine proteinkinase and an effector of the small GTPase Rho. With N-terminus protein kinase domainand C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can beactivated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho proteinbinds to the Rho-binding domain and induces a conformational change which opens thekinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIMkinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1,Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitoryc-terminal domain. This activation, independent of Rho activity, leads to apoptoticmembrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly,cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth.Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1an ideal target for drug development (3). Alternate Names: anti-MGC131603 antibody, anti-MGC43611 antibody, anti-NY REN 35 antigen antibody,anti-p160 ROCK1 antibody, anti-p160ROCK antibody, anti-Renal carcinoma antigen NYREN 35 antibody, anti-Rho associated coiled coil containing protein kinase 1 antibody,anti-Rho associated protein kinase 1 antibody.
Gene Symbol: ROCK1
|Gene ID||6093, 19877, 81762|
|Research Area||Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling|
|Pathways||Microtubule Dynamics, WNT Signaling|
Application DetailsProduct Details anti-ROCK1 Antibody Target Details ROCK1 Handling Images back to top
In Western blot this antibody detects a bands at approximately 30 & 160 kDa, corresponding to both C-terminal cleaved fragment and full length ROCK1. Immunohidtochemistry Frozen reported in literature (PMID: 19295659)
Recommended dilutions: Flow Cytometry 1:1000, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:100, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol specific for ROCK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling back to top