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anti-Rabbit ROCK1 antibody for Western Blotting

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • ROCK1
  • LOC100229794
  • rok
  • Rock1
  • rock1
Epitope
C-Term, cleaved
27
26
24
20
15
14
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
149
115
108
24
20
19
5
4
4
2
1
Host
Mouse
111
40
Clonality
Monoclonal
Conjugate
This ROCK1 antibody is un-conjugated
5
4
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
110
36
32
31
24
23
21
12
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN252549
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN2706968 IC IF WB Rabbit Center Log in to see Polyclonal
2.9590726 ABIN4350918 ICC IF WB Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350894 ICC IF WB Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350903 ICC IF WB DyLight 650 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350910 ICC IF WB DyLight 550 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350915 ICC IF WB Alexa Fluor 488 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350908 WB PerCP Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350917 ICC IF WB Alexa Fluor 700 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350911 ICC IF WB DyLight 350 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350914 ICC IF WB Alexa Fluor 405 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350912 ICC IF WB DyLight 405 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350905 ICC IF WB DyLight 488 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350916 ICC IF WB Alexa Fluor 647 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350902 ICC IF WB Biotin Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350904 ICC IF WB DyLight 680 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350907 ICC IF WB DyLight 755 Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350906 WB HRP Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350909 WB PE Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN4350901 WB Allophycocyanin Mouse IgG C-Term, cleaved Log in to see 154C1465
1 ABIN2627633 ICC IF WB Rabbit Internal Region Log in to see Polyclonal

Top referenced anti-ROCK1 antibody for Western Blotting

Similar anti-ROCK1 Antibodies

Application / Reactivity Mouse (Murine) Human Rabbit Rat (Rattus) Dog (Canine) Primate
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 20 Antibodies 109 Antibodies 77 Antibodies 24 Antibodies 70 Antibodies 19 Antibodies
Immunoprecipitation (IP) 11 Antibodies 8 Antibodies 5 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 16 Antibodies 15 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 14 Antibodies 11 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope C-Term, cleaved
(27), (26), (24), (20), (15), (14), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
(149), (115), (108), (24), (20), (19), (5), (4), (4), (2), (1)
Host Mouse
(111), (40)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK1 antibody is un-conjugated
(5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(110), (36), (32), (31), (24), (23), (21), (12), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN252549) Images
Specificity This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Purification Protein G purified
Immunogen This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Clone 154C1465
Isotype IgG

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 Antibody (ABIN252549) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background Gene Symbol: ROCK1
Gene ID 6093
UniProt O60259
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling, M Phase

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 Antibody (ABIN252549) Images back to top
Application Notes Western Blot 1-2 μg/mL, Immunocytochemistry/ImmunofluorescenceUse in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21059388)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 Antibody (ABIN252549) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-ROCK1 Antibody (ABIN252549)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Product cited in:

Goetsch, Kallmeyer, Niesler: "Decorin modulates collagen I-stimulated, but not fibronectin-stimulated, migration of C2C12 myoblasts." in: Matrix biology : journal of the International Society for Matrix Biology, Vol. 30, Issue 2, pp. 109-17, 2011 Further details: Immunocytochemistry,Immunofluorescence

Orlando, Pittman: "Rho kinase regulates phagocytosis, surface expression of GlcNAc, and Golgi fragmentation of apoptotic PC12 cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3298-311, 2006

Orlando, Stone, Pittman: "Rho kinase regulates fragmentation and phagocytosis of apoptotic cells." in: Experimental cell research, Vol. 312, Issue 1, pp. 5-15, 2005 Further details: Western Blotting

Zhao, Miller, Pfeiffer, Buras, Stahl: "Anoxia and reoxygenation of human endothelial cells decrease ceramide glucosyltransferase expression and activates caspases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 6, pp. 723-4, 2003 Further details: Western Blotting

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 Antibody (ABIN252549) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (C-Term) (ABIN252549) Detection of cleaved ROCK-1 in apoptotic Jurkat cell lysates (10 ug/lane). Lane 1. Ju...
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (C-Term) (ABIN252549) Western Blot: cleaved ROCK1 Antibody (154C1465) [ABIN2525496] - Analysis using Azide ...