anti-Rat (Rattus) ROCK1 antibody for Western Blotting

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • ROCK1
  • LOC100229794
  • rok
  • Rock1
  • rock1
Epitope
C-Term, cleaved
39
27
27
24
20
15
5
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
195
143
132
24
20
19
6
5
4
2
1
Host
Mouse
115
82
Clonality
Monoclonal
Conjugate
This ROCK1 antibody is un-conjugated
8
7
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
154
45
36
33
27
25
24
16
2
2
1
1
1
1
Supplier
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Catalog No. ABIN252549
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN968386 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
3.2182703 ABIN968387 IF WB Mouse IgG1 AA 906-1012 Log in to see 46-ROCK 5
3.2182703 ABIN1874629 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN3186829 ELISA ICC IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.2182703 ABIN2706968 IC IF WB Rabbit Center Log in to see Polyclonal
3.2182703 ABIN2737481 WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN4350897 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN4350919 WB Mouse IgG1 N-Term Log in to see 261
3.2182703 ABIN4350920 WB Mouse IgG2b N-Term Log in to see 464
3.2182703 ABIN4350918 ICC IF WB Mouse IgG C-Term, cleaved Log in to see 154C1465
3.2182703 ABIN2856887 ICC IF IP IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN4350898 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN1844979 WB Rabbit Log in to see Polyclonal
1 ABIN707771 IF (p) IHC (p) WB Rabbit IgG pThr455, pSer456 Log in to see Polyclonal 2
1 ABIN738636 IF (p) IHC (p) WB Rabbit IgG AA 980-1030 Log in to see Polyclonal 1
1 ABIN207918 WB Mouse IgG AA 1114-1126 Log in to see 154C1465
1 ABIN5554278 EIA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1104014 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN707780 IHC (p) WB HRP Rabbit IgG pThr455, pSer456 Log in to see Polyclonal

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope C-Term, cleaved
(39), (27), (27), (24), (20), (15), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
(195), (143), (132), (24), (20), (19), (6), (5), (4), (2), (1)
Host Mouse
(115), (82)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK1 antibody is un-conjugated
(8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(154), (45), (36), (33), (27), (25), (24), (16), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 antibody (ABIN252549) Images
Specificity This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Purification Protein G purified
Immunogen This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Clone 154C1465
Isotype IgG

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 antibody (ABIN252549) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background Gene Symbol: ROCK1
Gene ID 6093
UniProt O60259
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 antibody (ABIN252549) Images back to top
Application Notes Western Blot 1-2 μg/mL, Immunocytochemistry/ImmunofluorescenceUse in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21059388)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 antibody (ABIN252549) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-ROCK1 antibody (ABIN252549)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Product cited in:

Goetsch, Kallmeyer, Niesler: "Decorin modulates collagen I-stimulated, but not fibronectin-stimulated, migration of C2C12 myoblasts." in: Matrix biology : journal of the International Society for Matrix Biology, Vol. 30, Issue 2, pp. 109-17, 2011 Further details: Immunocytochemistry,Immunofluorescence

Orlando, Pittman: "Rho kinase regulates phagocytosis, surface expression of GlcNAc, and Golgi fragmentation of apoptotic PC12 cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3298-311, 2006

Orlando, Stone, Pittman: "Rho kinase regulates fragmentation and phagocytosis of apoptotic cells." in: Experimental cell research, Vol. 312, Issue 1, pp. 5-15, 2005 Further details: Western Blotting

Zhao, Miller, Pfeiffer, Buras, Stahl: "Anoxia and reoxygenation of human endothelial cells decrease ceramide glucosyltransferase expression and activates caspases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 6, pp. 723-4, 2003 Further details: Western Blotting

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 antibody (ABIN252549) back to top
Supplier Images
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (C-Term), (cleaved) antibody (ABIN252549) Detection of cleaved ROCK-1 in apoptotic Jurkat cell lysates (10 ug/lane). Lane 1. Ju...
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (C-Term), (cleaved) antibody (ABIN252549) Western Blot: cleaved ROCK1 Antibody (154C1465) [ABIN2525496] - Analysis using Azide ...