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anti-Human IRAK1 antibody for Enzyme Immunoassay

Recommended IRAK1 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
  • AA408924
  • Il1rak
  • IRAK
  • IRAK-1
  • IRAK1
  • IRAK1-S
  • mPLK
  • pelle
  • Plpk
  • RGD1563841
Epitope
C-Term
39
37
22
22
18
15
14
13
13
13
13
9
8
7
7
6
5
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
238
111
78
Host
Rabbit
221
46
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
13
11
10
6
6
6
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
191
124
69
44
43
40
29
19
16
13
8
3
2
Supplier
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Catalog No. ABIN360116
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN401038 EIA IF IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 5
0.0008833851 ABIN500036 EIA IF IP WB Rabbit IgG C-Term Log in to see Polyclonal 5
0.0008833851 ABIN492547 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 1F12C1
0.0008833851 ABIN492548 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 8F1A7
0.0008833851 ABIN303104 EIA IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008833851 ABIN614624 EIA IF IHC (p) WB Mouse IgG2a AA 530-693 Log in to see 3F7
0.0008833851 ABIN614625 EIA IF IHC (p) IP WB Mouse IgG1 AA 530-693 Log in to see 3A9
0.0008833851 ABIN615177 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-IRAK1 antibody for Enzyme Immunoassay

Similar anti-IRAK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
ELISA 109 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 69 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 40 Antibodies
RNA Interference (RNAi) 3 Antibodies
Western Blotting (WB) 172 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
Epitope C-Term
(39), (37), (22), (22), (18), (15), (14), (13), (13), (13), (13), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(238), (111), (78)
Host Rabbit
(221), (46), (2)
Clonality
Polyclonal
Conjugate This IRAK1 antibody is un-conjugated
(13), (11), (10), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(191), (124), (69), (44), (43), (40), (29), (19), (16), (13), (8), (3), (2)
Pubmed 4 references available
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Product Details anti-IRAK1 Antibody

Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN360116) Images
Specificity This antibody reacts to IRAK.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human IRAK1.
Isotype Ig

Target Details IRAK1

Product Details anti-IRAK1 Antibody Application Details Handling References for anti-IRAK1 Antibody (ABIN360116) Images back to top
Antigen
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654, 9606
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-IRAK1 Antibody Target Details IRAK1 Handling References for anti-IRAK1 Antibody (ABIN360116) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details References for anti-IRAK1 Antibody (ABIN360116) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-IRAK1 Antibody (ABIN360116)

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling Images back to top
Background publications

Jensen, Whitehead: "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1500-6, 2003

Jiang, Johnson, Nie, Qin, Bird, Li et al.: "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 ..." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 10952-6, 2003

Strelow, Kollewe, Wesche: "Characterization of Pellino2, a substrate of IRAK1 and IRAK4." in: FEBS letters, Vol. 547, Issue 1-3, pp. 157-61, 2003

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996

Images

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN360116) back to top
Supplier Images
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN360116) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN360116) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody (Image 2)