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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 14 (MAP3K14) Antibodies|
|Epitope||Middle Region Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This MAP3K14 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K14 AntibodyTarget Details MAP3K14 Application Details Handling References for anti-MAP3K14 antibody (ABIN360384) Images
|Specificity||This antibody reacts to NFkB Inducing Kinase (NIK).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NIK.|
Target Details MAP3K14Product Details anti-MAP3K14 Antibody Application Details Handling References for anti-MAP3K14 antibody (ABIN360384) Images back to top
|Alternative Name||MAP3K14 (MAP3K14 Antibody Abstract)|
|Background||NIK (mitogen-activated protein kinase kinase kinase 14), a member of the Ser/Thr protein kinase family, binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.Synonyms: Mitogen-activated protein kinase kinase kinase 14, NF-kappa beta-inducing kinase, Serine/threonine-protein kinase NIK|
|Research Area||Kinases/Phosphatases, Transcription Factors|
|Pathways||NF-kappaB Signaling, TCR Signaling|
Application DetailsProduct Details anti-MAP3K14 Antibody Target Details MAP3K14 Handling References for anti-MAP3K14 antibody (ABIN360384) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details References for anti-MAP3K14 antibody (ABIN360384) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP3K14 antibody (ABIN360384)Product Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details Handling Images back to top
Andreakos, Smith, Monaco, Brennan, Foxwell, Feldmann: "Ikappa B kinase 2 but not NF-kappa B-inducing kinase is essential for effective DC antigen presentation in the allogeneic mixed lymphocyte reaction." in: Blood, Vol. 101, Issue 3, pp. 983-91, 2003
Ninomiya-Tsuji, Kishimoto, Hiyama, Inoue, Cao, Matsumoto: "The kinase TAK1 can activate the NIK-I kappaB as well as the MAP kinase cascade in the IL-1 signalling pathway." in: Nature, Vol. 398, Issue 6724, pp. 252-6, 1999
Aronsson, Magnusson, Andersson, Karsten, Shibasaki, Lendon, Goate, Brookes: "The NIK protein kinase and C17orf1 genes: chromosomal mapping, gene structures and mutational screening in frontotemporal dementia and parkinsonism linked to chromosome 17." in: Human genetics, Vol. 103, Issue 3, pp. 340-5, 1998
ImagesProduct Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details Handling References for anti-MAP3K14 antibody (ABIN360384) back to top