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anti-Human TNFRSF11A antibody for Flow Cytometry

Recommended TNFRSF11A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
  • CD265
  • FEO
  • LOH18CR1
  • Ly109
  • mRANK
  • ODFR
  • OFE
  • OPTB7
  • OSTS
  • PDB2
  • Rank
  • RANK
  • RGD1563614
  • TNFRSF11A
  • TRANCE-R
  • TRANCER
Epitope
AA 326-616, AA 330-427
16
13
10
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
58
28
19
Host
Mouse
47
19
8
Clonality
Monoclonal
Conjugate
This TNFRSF11A antibody is conjugated to PE
7
4
4
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
38
33
11
9
8
5
4
3
3
3
2
Supplier
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Available images

Catalog No. ABIN252036
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666476 ABIN2470154 FACS IHC WB Mouse IgG1 AA 326-616 Log in to see 9A725
0.0010666476 ABIN187381 FACS WB Mouse IgG1 AA 330-427 Log in to see 9A725

Similar anti-TNFRSF11A Antibodies

Application / Reactivity Human
ELISA 29 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Western Blotting (WB) 30 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
Epitope AA 326-616, AA 330-427
(16), (13), (10), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(58), (28), (19)
Host Mouse
(47), (19), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF11A antibody is conjugated to PE
(7), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(38), (33), (11), (9), (8), (5), (4), (3), (3), (3), (2)
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Product Details anti-TNFRSF11A Antibody

Target Details TNFRSF11A Application Details Handling Images
Specificity In RAW cells, a 97 kDa is observed.
Cross-Reactivity (Details) Not yet tested in other species.
Purification protein G purified Mouse ascites
Immunogen A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Clone 9A725
Isotype IgG1 kappa

Target Details TNFRSF11A

Product Details anti-TNFRSF11A Antibody Application Details Handling Images back to top
Antigen
Alternative Name RANK/TNFRSF11A (TNFRSF11A Antibody Abstract)
Background Apoptosis, or programmed cell death, occurs during normal cellular differentiation anddevelopment of multicellular organisms. Apoptosis is induced by certain cytokinesincluding TNF and Fas ligand of the TNF family through their death domain containingreceptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently clonedmember of the TNFR superfamily with no significant homology to other members of thisfamily (1). RANK ligand (RANKL/TRANCE/ OPGL) (mAbs to RANKL is also availablefrom Imgenex, Cat # IMG-133) binds to RANK on dendritic cells, upregulates theexpression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival(2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4).Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways(3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB,whereas JNK activation is mediated through binding of RANK to TRAF2 (4). Alternate Names: anti-TNF11aR antibody, anti-ODFR antibody, anti-Receptor Activator of NF-kB antibody.
Gene Symbol: TNFRSF11A
Gene ID 8792, 21934
NCBI Accession NP_003830
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Handling Images back to top
Application Notes Bharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry.
Recommended dilutions: Flow (Cell Surface), Flow Cytometry 0.625 µg/10^6 cells, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Western Blot 1:100-1:2000
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Storage Comment 4 °C in the dark

Images

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (PE) (ABIN252036) Flow Cytometry: RANK Antibody (9A725) [ABIN252036] - Flow cytometry analysis of RANK ...