anti-Human BBC3 antibody for Immunofluorescence

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
C-Term, AA 150-193
94
62
18
18
16
12
10
9
9
8
5
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
290
122
107
18
1
1
1
Host
Rabbit
259
24
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
274
86
76
59
49
32
20
13
8
7
2
1
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Catalog No. ABIN153058
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.177332 ABIN4348684 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 4
11.177332 ABIN259765 ICC IF WB Rabbit AA 1-100, Isoform 1, N-Term Log in to see Polyclonal
11.177332 ABIN4348685 ELISA ICC IF WB Rabbit Log in to see Polyclonal
11.177332 ABIN3030112 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 119-154 Log in to see Polyclonal
1 ABIN388112 FACS IF IHC (p) WB Rabbit Ig AA 119-154, BH3 Domain Log in to see Polyclonal 1
1 ABIN356830 EIA FACS IF IHC (p) WB Rabbit Ig BH3 Domain, C-Term Log in to see Polyclonal 3
1 ABIN223064 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN361748 ICC IF WB Rabbit C-Term Log in to see Polyclonal 3
1 ABIN5551336 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5555150 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5555151 EIA IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2485944 ICC IF WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485945 ICC IF WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485946 ICC IF WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485947 ICC IF WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485948 ICC IF WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485949 ICC IF WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485950 ICC IF WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485951 ICC IF WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485952 ICC IF WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope C-Term, AA 150-193
(94), (62), (18), (18), (16), (12), (10), (9), (9), (8), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(290), (122), (107), (18), (1), (1), (1)
Host Rabbit
(259), (24), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(274), (86), (76), (59), (49), (32), (20), (13), (8), (7), (2), (1)
Pubmed 2 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN153058) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193). [UniProt# Q9BXH1]

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN153058) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Gene Symbol: BBC3
Molecular Weight Theoretical MW: 16 kDa
Gene ID 27113
UniProt Q9BXH1
Research Area Metabolism, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling ProductDetails: References for anti-BBC3 antibody (ABIN153058) Images back to top
Application Notes Western Blot 2-4 μg/mL, Immunocytochemistry/Immunofluorescence 1:20-1:100This PUMA antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By WB a band at ~16 kDa is seen, representing the beta form of PUMA. Reactivity against the alpha form of PUMA is unknown. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PUMA Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 35 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-PUMA primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details ProductDetails: References for anti-BBC3 antibody (ABIN153058) Images back to top
Format Liquid
Concentration 5.65 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-BBC3 antibody (ABIN153058)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Product cited in:

Stylianou, Clarke, Brennan: "Aberrant activation of notch signaling in human breast cancer." in: Cancer research, Vol. 66, Issue 3, pp. 1517-25, 2006 (Sample species: Human). Further details: Western Blotting

Yu, Wang, Kinzler, Vogelstein, Zhang: "PUMA mediates the apoptotic response to p53 in colorectal cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 1931-6, 2003

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN153058) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) (AA 150-193), (C-Term) antibody (ABIN153058) Detection of the beta isoform of PUMA in HL-60 whole cell lysate using ABIN153058(4 u...
Immunofluorescence (IF) image for anti-BCL2 Binding Component 3 (BBC3) (AA 150-193), (C-Term) antibody (ABIN153058) Immunocytochemistry/Immunofluorescence: PUMA Antibody [ABIN153058] - The PUMA antibod...