anti-Human BBC3 antibody for Western Blotting

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
AA 119-154, BH3 Domain
93
62
19
18
16
11
10
9
9
8
5
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
293
120
107
18
1
1
1
Host
Rabbit
260
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
276
86
77
60
48
31
19
13
8
7
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN388112
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0855882 ABIN153058 ICC IF WB Rabbit C-Term, AA 150-193 Log in to see Polyclonal 2
3.0855882 ABIN411574 IHC WB Rabbit IgG Log in to see Polyclonal
3.0855882 ABIN2705162 WB Rabbit C-Term Log in to see Polyclonal
3.0855882 ABIN658021 IHC (p) WB Rabbit Ig AA 109-137, Center Log in to see Polyclonal 1
3.0855882 ABIN4348682 ELISA WB Goat Isoform 1 Log in to see Polyclonal 1
3.0855882 ABIN731528 FACS IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 2
3.0855882 ABIN252347 IHC (p) IHC WB Rabbit Log in to see Polyclonal 5
3.0855882 ABIN153032 WB Rabbit Log in to see Polyclonal 3
3.0855882 ABIN4348684 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 4
3.0855882 ABIN1031530 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 1
3.0855882 ABIN259765 ICC IF WB Rabbit AA 1-100, Isoform 1, N-Term Log in to see Polyclonal
3.0855882 ABIN3186602 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
3.0855882 ABIN2787887 WB Rabbit N-Term Log in to see Polyclonal
3.0855882 ABIN2485944 ICC IF WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485946 ICC IF WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485949 ICC IF WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485951 ICC IF WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485950 ICC IF WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485953 ICC IF WB APC Rabbit C-Term Log in to see Polyclonal 3
3.0855882 ABIN2485957 ICC IF WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope AA 119-154, BH3 Domain
(93), (62), (19), (18), (16), (11), (10), (9), (9), (8), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(293), (120), (107), (18), (1), (1), (1)
Host Rabbit
(260), (24), (15)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(276), (86), (77), (60), (48), (31), (19), (13), (8), (7), (2), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN388112) Images
Specificity This antibody was developed against a synthetic peptide selected from the C-terminal region of human Puma.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 AA from human Puma BH3 Domain.
Clone RB01353
Isotype Ig

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 antibody (ABIN388112) Images back to top
Antigen
Alternative Name Puma (BBC3 Antibody Abstract)
Background Synonyms: Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, JFY-1, BBC3
Molecular Weight 20532 DA
Gene ID 27113
UniProt Q9BXH1
Research Area Apoptosis/Necrosis, Cancer, Transcription Factors, Signaling
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 antibody (ABIN388112) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 antibody (ABIN388112) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BBC3 antibody (ABIN388112)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Product cited in:

Vesela, Matalova: "Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, pp. 1-7, 2015

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN388112) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) (AA 119-154), (BH3 Domain) antibody (ABIN388112) Western blot analysis of anti-Puma BH3 domain Pab (ABIN388112) in HL-60 cell lysate. ...
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) (AA 119-154), (BH3 Domain) antibody (ABIN388112) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) (AA 119-154), (BH3 Domain) antibody (ABIN388112) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma ...