anti-Human BBC3 antibody for Western Blotting

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
AA 109-137, Center
95
62
19
18
16
12
9
9
8
8
5
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
290
123
107
18
1
1
1
Host
Rabbit
259
24
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
274
86
76
60
51
31
20
13
9
7
2
1
Options
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Catalog No. ABIN658021
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.054504 ABIN388112 FACS IF IHC (p) WB Rabbit Ig AA 119-154, BH3 Domain Log in to see Polyclonal 1
3.054504 ABIN153058 ICC IF WB Rabbit C-Term, AA 150-193 Log in to see Polyclonal 2
3.054504 ABIN4348682 ELISA WB Goat Isoform 1 Log in to see Polyclonal 1
3.054504 ABIN731528 FACS IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 2
3.054504 ABIN2787887 WB Rabbit N-Term Log in to see Polyclonal
3.054504 ABIN4348684 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 4
3.054504 ABIN153032 WB Rabbit Log in to see Polyclonal 3
3.054504 ABIN252347 IHC (p) IHC WB Rabbit Log in to see Polyclonal 5
3.054504 ABIN2485944 ICC IF WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485945 ICC IF WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485946 ICC IF WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485947 ICC IF WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485948 ICC IF WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485949 ICC IF WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485950 ICC IF WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485951 ICC IF WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485952 ICC IF WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485953 ICC IF WB APC Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485954 ICC IF WB Biotin Rabbit C-Term Log in to see Polyclonal 3
3.054504 ABIN2485955 ICC IF WB FITC Rabbit C-Term Log in to see Polyclonal 3

Top referenced anti-BBC3 antibody for Western Blotting

Similar anti-BBC3 Antibodies

Application / Reactivity Human
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 51 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 268 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope AA 109-137, Center
(95), (62), (19), (18), (16), (12), (9), (9), (8), (8), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(290), (123), (107), (18), (1), (1), (1)
Host Rabbit
(259), (24), (14)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(274), (86), (76), (60), (51), (31), (20), (13), (9), (7), (2), (1)
Pubmed 1 reference available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN658021) Images
Specificity This BBC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the Central region of human BBC3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BBC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 AA from the Central region of human BBC3.
Clone RB33714
Isotype Ig

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN658021) Images back to top
Antigen
Alternative Name BBC3 (BBC3 Antibody Abstract)
Background BBC3 is an essential mediator of p53-dependent and p53-independent apoptosis.
Synonyms: Bcl-2-binding component 3,BBC3,PUMA
Molecular Weight 20532 DA
Gene ID 27113
NCBI Accession NP_055232, NP_001120714, NP_001120713
UniProt Q9BXH1
Research Area Apoptosis/Necrosis, Cell Structure
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling ProductDetails: References for anti-BBC3 antibody (ABIN658021) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details ProductDetails: References for anti-BBC3 antibody (ABIN658021) Images back to top
Format Liquid
Concentration 0.45 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment BBC3 Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months

ProductDetails: References for anti-BBC3 antibody (ABIN658021)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Background publications

Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN658021) back to top
Supplier Images
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (AA 109-137) (ABIN658021) BBC3 Antibody (Center) (ABIN658021) western blot analysis in A549 cell line lysates (...