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anti-Mouse (Murine) BBC3 antibody for Flow Cytometry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Epitope
BH3 Domain, C-Term
95
52
21
15
13
13
12
11
8
3
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
273
79
58
2
1
1
Host
Rabbit
241
28
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
13
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
260
84
65
52
42
32
11
8
5
4
3
2
2
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Catalog No. ABIN356830
$ 390.50
Plus shipping costs $45.00

Top referenced anti-BBC3 antibody for Flow Cytometry

Similar anti-BBC3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 15 Antibodies 79 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 9 Antibodies
Flow Cytometry (FACS) 1 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 26 Antibodies 54 Antibodies
Immunofluorescence (IF) 30 Antibodies 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 70 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope BH3 Domain, C-Term
(95), (52), (21), (15), (13), (13), (12), (11), (8), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(273), (79), (58), (2), (1), (1)
Host Rabbit
(241), (28), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(13), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(260), (84), (65), (52), (42), (32), (11), (8), (5), (4), (3), (2), (2)
Pubmed 3 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN356830) Images
Specificity This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen Synthetic peptide selected from the C-terminal region of Human PUMA
Isotype Ig

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 Antibody (ABIN356830) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Molecular Weight 20532 Da
Gene ID 27113, 9606
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 Antibody (ABIN356830) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 Antibody (ABIN356830) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BBC3 Antibody (ABIN356830)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Background publications

Hoque, Begum, Sommer, Lee, Trink, Ratovitski, Sidransky: "PUMA in head and neck cancer." in: Cancer letters, Vol. 199, Issue 1, pp. 75-81, 2003

Yu, Wang, Kinzler, Vogelstein, Zhang: "PUMA mediates the apoptotic response to p53 in colorectal cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 1931-6, 2003

Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN356830) back to top
Supplier Images
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (BH3 Domain) (ABIN356830) anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (BH3 Domain) (ABIN356830) anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody (Image 2)
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (BH3 Domain) (ABIN356830) anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody (Image 3)
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (BH3 Domain) (ABIN356830) anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody (Image 4)