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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||13 Antibodies||73 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||9 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||7 Antibodies|
|Immunocytochemistry (ICC)||24 Antibodies||46 Antibodies|
|Immunofluorescence (IF)||28 Antibodies||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||64 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||BCL2 Binding Component 3 (BBC3) Antibodies|
|Epitope||BH3 Domain, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This BBC3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-BBC3 AntibodyTarget Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN356830) Images
|Specificity||This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.|
|Purification||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||Synthetic peptide selected from the C-terminal region of Human PUMA|
Target Details BBC3Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 Antibody (ABIN356830) Images back to top
|Alternative Name||PUMA (BBC3 Antibody Abstract)|
|Background||PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis|
|Molecular Weight||20532 Da|
|Gene ID||27113, 9606|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
Application DetailsProduct Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 Antibody (ABIN356830) Images back to top
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 Antibody (ABIN356830) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-BBC3 Antibody (ABIN356830)Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003 (PubMed).
Hoque, Begum, Sommer et al.: "PUMA in head and neck cancer." in: Cancer letters, Vol. 199, Issue 1, pp. 75-81, 2003 (PubMed).
Yu, Wang, Kinzler et al.: "PUMA mediates the apoptotic response to p53 in colorectal cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 1931-6, 2003 (PubMed).
ImagesProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN356830) back to top