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anti-Rat (Rattus) BBC3 antibody for Enzyme Immunoassay

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Epitope
AA 76-170
88
50
21
13
13
13
11
7
7
3
3
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
254
74
54
2
1
1
Host
Mouse
227
24
13
Clonality
Monoclonal
Conjugate
This BBC3 antibody is un-conjugated
11
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
241
78
62
44
35
30
11
8
6
4
3
2
2
Supplier
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Catalog No. ABIN492517
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088294124 ABIN492519 EIA WB Mouse IgG1 AA 76-170 Log in to see 10C5G1
0.00088294124 ABIN492518 EIA WB Mouse IgG1 AA 76-170 Log in to see 2A9G5
0.00088294124 ABIN492520 EIA WB Mouse IgG1 AA 76-170 Log in to see 10D4G7

Similar anti-BBC3 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 24 Antibodies 73 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 9 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 46 Antibodies
Immunofluorescence (IF) 2 Antibodies 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 42 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope AA 76-170
(88), (50), (21), (13), (13), (13), (11), (7), (7), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(254), (74), (54), (2), (1), (1)
Host Mouse
(227), (24), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BBC3 antibody is un-conjugated
(11), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(241), (78), (62), (44), (35), (30), (11), (8), (6), (4), (3), (2), (2)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Specificity Recognizes Human and Rat PUMA. Other species not tested.
Characteristics Synonyms: BBC3, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis
Purification Immunoaffinity Chromotography
Immunogen A recombinant protein corresponding to amino acids 76–170 of Human PUMA-alpha
Clone 2A8F6
Isotype IgG1

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Gene ID 27113
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes ELISA. Western Blot: PUMA antibody can be used for detection of PUMA at 2.5-5 μg/mL. Positive Control: K562 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (AA 76-170) (ABIN492517) anti-BCL2 Binding Component 3 (BBC3) (AA 76-170) antibody