anti-Rat (Rattus) BBC3 antibody for Immunohistochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
N-Term
94
61
19
18
16
12
10
9
9
8
5
5
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Rainbow Trout (Oncorhynchus mykiss), Rat (Rattus)
294
122
106
17
1
1
1
Host
Rabbit
262
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is conjugated to Atto 390
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
278
85
77
60
50
32
20
13
8
7
2
1
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Catalog No. ABIN5024197
$ 458.54
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.869368 ABIN5024200 IHC WB Atto 488 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024206 IHC WB Atto 594 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024212 IHC WB Atto 655 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024221 IHC WB Alkaline Phosphatase (AP) Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024224 IHC WB APC Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024227 IHC WB Biotin Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024233 IHC WB HRP Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024239 IHC WB PerCP Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024245 IHC WB Streptavidin Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024242 IHC WB PE Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024215 IHC WB Atto 680 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024203 IHC WB Atto 565 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024209 IHC WB Atto 633 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024218 IHC WB Atto 700 Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024230 IHC WB FITC Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN5024236 IHC WB PE-Atto 594 Rabbit N-Term Log in to see Polyclonal
1 ABIN411574 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN361754 IHC WB Rabbit N-Term Log in to see Polyclonal 4
1 ABIN4965576 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1870134 ICC IHC IP WB Rabbit IgG AA 76-170 Log in to see Polyclonal

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope N-Term
(94), (61), (19), (18), (16), (12), (10), (9), (9), (8), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rainbow Trout (Oncorhynchus mykiss), Rat (Rattus)
(294), (122), (106), (17), (1), (1), (1)
Host Rabbit
(262), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is conjugated to Atto 390
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(278), (85), (77), (60), (50), (32), (20), (13), (8), (7), (2), (1)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Specificity Detects the N-terminal domain of PUMA ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
Purification Protein A Purified
Immunogen N-terminal amino acids of human PUMA

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Gene ID 27114
NCBI Accession NP_001120712
UniProt Q9BXH2
Research Area Apoptosis/Necrosis, Metabolism, Cancer
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes
  • WB (1:500)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SPC-165 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Concentration 1 mg/mL
Buffer PBS, 0.02 % sodium azide
Storage 4 °C

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Atto 390) (ABIN5024197) Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: br...
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Atto 390) (ABIN5024197) Western blot analysis of Human K562 cells lysates showing detection of PUMA protein u...
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Atto 390) (ABIN5024197) Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: co...