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anti-Rat (Rattus) BBC3 antibody for Immunohistochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
287
84
60
1
1
1
Host
Rabbit
253
28
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
13
11
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
268
84
67
61
52
34
18
11
9
5
3
2
2
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Available images

Catalog No. ABIN411574
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2467757 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN4965576 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2425609 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN4903014 FACS ICC IF IHC WB Rabbit IgG Log in to see

Similar anti-BBC3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 263 Antibodies 71 Antibodies 47 Antibodies
Immunoprecipitation (IP) 5 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies 12 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 68 Antibodies 9 Antibodies 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(287), (84), (60), (1), (1), (1)
Host Rabbit
(253), (28), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(13), (11), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(268), (84), (67), (61), (52), (34), (18), (11), (9), (5), (3), (2), (2)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN412890
Specificity The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 177 of human PUMA
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms: PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3
Gene ID 27113
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunohistochemistry (5 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Suggested positive control: Western Blot: Jurkat cells and Rat kidney tissue, IHC: Tonsil tissue
Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN411574) anti-BCL2 Binding Component 3 (BBC3) antibody