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anti-Human C-MYC antibody for Fluorescence Microscopy

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • AU016757
  • bHLHe39
  • c-myc
  • c-Myc
  • C-MYC
  • cmyc
  • CMYC
  • CMyc
  • LOC100136746
  • mMyc
  • MRTL
  • MYC
  • Myc2
  • MYCC
  • Niard
  • Nird
  • RNCMYC
  • zc-myc
Reactivity
Human
638
189
151
10
9
6
5
5
4
3
3
3
3
3
2
1
1
1
1
1
Host
Mouse
426
201
16
13
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
40
32
28
15
14
12
4
4
4
4
4
4
4
4
4
3
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
428
230
199
114
104
91
71
44
41
36
21
11
10
10
7
6
4
4
2
2
1
Supplier
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Catalog No. ABIN967319
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN967320 FM IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 9E10 13

Top referenced anti-C-MYC antibody for Fluorescence Microscopy

Similar anti-C-MYC Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 4 Antibodies
Dot Blot (DB) 36 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antibodies
ELISA 212 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 72 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunofluorescence (IF) 113 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 197 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Reactivity Human
(638), (189), (151), (10), (9), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(426), (201), (16), (13), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(40), (32), (28), (15), (14), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(428), (230), (199), (114), (104), (91), (71), (44), (41), (36), (21), (11), (10), (10), (7), (6), (4), (4), (2), (2), (1)
Pubmed 13 references available
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN967319) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human c-Myc Peptide
Clone 9E10
Isotype IgG1

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC Antibody (ABIN967319) Images back to top
Antigen
Alternative Name c-Myc (MYC Antibody Abstract)
Background The myc gene family contains at least seven closely related genes, c-myc, N-myc, L-myc, P-myc, R-myc, S-myc, and B-myc. C-myc plays a role in proliferation, transformation, and differentiation. It is expressed during embryonic development, in a wide variety of adult tissues, and is amplified in many tumors, particularly lung, breast, cervical and colon carcinomas. Cellular localization has been described as nuclear and/or cytopasmic. C-myc has three sequence motifs in the carboxy terminus, a leucine zipper, a helix-loop-helix, and a basic region. It forms sequence-specific (CACGTG) DNA binding heterodimers with max, a helixloop-helix/leucine zipper protein. Both the leucine zipper domain and the helixloop-helix motif of c-myc contribute to heterodimer formation. DNA binding of myc-max dimers results in a conformational change of the DNA and transcriptional activation. C-myc migrates at 62 kDa in SDS-PAGE. Clone 9E10 recognizes human c-myc. A synthetic peptide corresponding to a C-terminal epitope of the human c-myc protein (AEEQKLISEEDL) was used as an immunogen.
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC Antibody (ABIN967319) Images back to top
Application Notes It is particularly useful for western blot analysis of myc-tagged recombinant proteins, and recombinant c-myc. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20 °C). Additional control lysate is sold separately.
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC Antibody (ABIN967319) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-C-MYC Antibody (ABIN967319)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Background publications

Liu, Hsu, Goeddel, Karin: "Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death." in: Cell, Vol. 87, Issue 3, pp. 565-76, 1996

Lukas, Parry, Aagaard, Mann, Bartkova, Strauss, Peters, Bartek: "Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16." in: Nature, Vol. 375, Issue 6531, pp. 503-6, 1995

Campbell, Kessler, Fambrough: "The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9321-5, 1992

Royds, Sharrard, Wagner, Polacarz: "Cellular localisation of c-myc product in human colorectal epithelial neoplasia." in: The Journal of pathology, Vol. 166, Issue 3, pp. 225-33, 1992

Tosca, Linardopoulos, Malliri, Hatziolou, Nicolaidou, Spandidos: "Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides." in: Anticancer research, Vol. 11, Issue 4, pp. 1433-8, 1992

Blackwood, Eisenman: "Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc." in: Science (New York, N.Y.), Vol. 251, Issue 4998, pp. 1211-7, 1991

DePinho, Schreiber-Agus, Alt: "myc family oncogenes in the development of normal and neoplastic cells." in: Advances in cancer research, Vol. 57, pp. 1-46, 1991

Gosney, Field, Gosney, Lye, Spandidos, Butt: "c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types." in: Anticancer research, Vol. 10, Issue 3, pp. 623-8, 1990

Karayiannis, Yiagnisis, Papadimitriou, Field, Spandidos: "Evaluation of the ras and myc oncoproteins in benign gastric lesions." in: Anticancer research, Vol. 10, Issue 5A, pp. 1127-33, 1990

Spandidos, Yiagnisis, Papadimitriou, Field: "ras, c-myc and c-erbB-2 oncoproteins in human breast cancer." in: Anticancer research, Vol. 9, Issue 5, pp. 1385-93, 1990

Williams, Piris, Wyllie: "Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms." in: The Journal of pathology, Vol. 160, Issue 4, pp. 287-93, 1990

Tiniakos, Spandidos, Kakkanas, Pintzas, Pollice, Tiniakos: "Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues." in: Anticancer research, Vol. 9, Issue 3, pp. 715-21, 1989

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN967319) back to top
Supplier Images
Western Blotting (WB) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (ABIN967319) Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (...