HVEM Protein (AA 39-184) (Fc Tag)

Catalog No. ABIN2666456

Product Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 39-184

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This HVEM protein is labelled with Fc Tag.

Application: Biochemical Assay (BCA)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human HVEM-Fc is able to neutralize 0.25 ng/ml of human TNF-β induced cytotoxicity on murine L929 cells. The ED 50 is 1.3 - 1.9 μg/ml, corresponding to a specific activity of 5.26 x 102 - 7.69 x 102 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store in working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: HVEM (TNFRSF14 Products)

Synonyms: ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein

Target Type: Viral Protein

Background: Herpes virus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor family, mediates herpes virus entry into cells during infection. Upon overexpression, HVEM activates NF-kappaB and AP-1 through a TNF receptor-associated factor (TRAF)-mediated mechanism. HVEM ligands belong to two distinct families: the TNF-related cytokines LIGHT and lymphotoxin-α, and the Ig-related membrane proteins BTLA and CD160. HVEM and its ligands have been involved in the pathogenesis of various autoimmune and inflammatory diseases, tumor progression and resistance to immune response. It has been demonstrated that HVEM induces a novel signaling pathway downstream leading to signal transduction and activation of STAT3 in epithelial cells. Since STAT3 regulates the expression of genes important for host defense in epithelial cells, as well as the differentiation of retinoid-related orphan receptor RORγt+, Th17, and innate lymphoid cells. This finding suggests that HVEM may play an important role in mucosal host defense.

Molecular Weight: The 376 amino acid recombinant protein consists of an N-terminal domain corresponding to the extracellular domain of HVEM and C-terminal portion corresponding to amino acid 102 to 330 of human IgG1. The protein has a predicted molecular mass of approximat

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints