Rpb1 CTD antibody (Tyr1)

Catalog No. ABIN2668519

Product Details anti-Rpb1 CTD Antibody

Target: Rpb1 CTD (RNA Polymerase II CTD)

Binding Specificity: Tyr1

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Clone: 3D12

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Rpb1 CTD antibody (ABIN2668519)

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Zaborowska, Baumli, Laitem, OReilly, Thomas, OHare, Murphy: "Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation." in: PLoS ONE, Vol. 9, Issue 9, pp. e107654, (2014) (PubMed).

Mayer, Heidemann, Lidschreiber, Schreieck, Sun, Hintermair, Kremmer, Eick, Cramer: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." in: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD (Rpb1 CTD Products)

Synonyms: 5 antibody, 8WG16 antibody, CG1554 antibody, CTD antibody, Dmel\\CG1554 antibody, H5 antibody, II antibody, L5 antibody, POL antibody, Pol II antibody, Pol II CTD antibody, Pol II Ser5P antibody, Pol II0[ser2] antibody, Pol II0[ser5] antibody, Pol II[ser2] antibody, Pol IIa antibody, Pol IIo antibody, Pol IIo[Ser2] antibody, Pol IIo[ser2] antibody, Pol IIo[ser5] antibody, Pol-IIa antibody, PolII antibody, PolIIo antibody, RNA Pol II antibody, RNA Pol II CTD antibody, RNA PolI 215 antibody, RNA PolII antibody, RNA pol II antibody, RNA pol IIo antibody, RNA polII antibody, RNAP antibody, RNAP II antibody, RNAP II LS antibody, RNAPII antibody, RNAPII0 antibody, RNApol2 antibody, RNApolII antibody, RPB1 antibody, RPII215 antibody, RpII antibody, Rpb1 antibody, Rpll215 antibody, Ubl antibody, dRPB1 antibody, dRpb1 antibody, l(1)10Ca antibody, l(1)DC912 antibody, l(1)DF912 antibody, l(1)G0040 antibody, l(1)L5 antibody, pol II antibody, polII antibody, RNA polymerase II 215kD subunit antibody, RpII215 antibody

Molecular Weight: 210 kDa

Gene ID: 5430