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anti-Cow (Bovine) GRB2 antibody for Immunofluorescence

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • AA408164
  • ASH
  • Ash
  • EGFRBP-GRB2
  • GRB2
  • grb2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
151
44
44
9
8
3
3
2
2
2
2
1
Host
Mouse
102
28
22
2
Clonality
Monoclonal
Conjugate
This GRB2 antibody is un-conjugated
5
4
3
3
3
3
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
133
67
53
29
8
8
5
3
3
2
1
Supplier
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Catalog No. ABIN180592
$ 565.95
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843406 ABIN267213 ICC IF IHC (p) IHC Mouse IgG1 kappa Log in to see 2GB04

Similar anti-GRB2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Xenopus laevis
Western Blotting (WB) 132 Antibodies 44 Antibodies 43 Antibodies 9 Antibodies
Immunoprecipitation (IP) 9 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 6 Antibodies 9 Antibodies 9 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 56 Antibodies 15 Antibodies 13 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(151), (44), (44), (9), (8), (3), (3), (2), (2), (2), (2), (1)
Host Mouse
(102), (28), (22), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GRB2 antibody is un-conjugated
(5), (4), (3), (3), (3), (3)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(133), (67), (53), (29), (8), (8), (5), (3), (3), (2), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Specificity This antibody detects GRB2 at aa 54-62
Characteristics Synonyms: Growth factor receptor-bound protein 2
Purification Protein G chromatography
Immunogen Purified recombinant human Grb2 protein
Clone 2GB04-same C1-4
Isotype IgG1

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 / ASH (GRB2 Antibody Abstract)
Background Grb2 protein contains one SH2 domain flanked on both sides by an SH3 domain. It binds to the junction between the amino terminal SH3 domain and the SH2 domain of Grb2. Reactivity with Grb2 deletion mutants suggests that its epitope maps between aa 54-62. In response to EGF, Grb2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The Grb2-Sos complex then binds the EGF-receptor. These interactions play a critical role in the activation of the p21ras signalling pathway.Synonyms: Growth factor receptor-bound protein 2
Gene ID 2885
UniProt P62993
Research Area Signaling
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Immunofluorescence. Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HeLa cells. Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (ABIN180592) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody