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anti-Mouse (Murine) GRB2 antibody for Western Blotting

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • AA408164
  • ASH
  • Ash
  • EGFRBP-GRB2
  • GRB2
  • grb2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
Epitope
C-Term
34
18
15
7
6
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
148
42
41
10
8
3
3
2
2
2
2
1
1
Host
Goat
99
29
21
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
5
4
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
129
64
52
30
9
8
5
3
3
2
1
Supplier
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Catalog No. ABIN184670
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.125708 ABIN2853618 WB Mouse IgG1 N-Term Log in to see 3F2 6
0.125708 ABIN1532105 IHC ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal 2
0.125708 ABIN1534949 ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.125708 ABIN967754 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
0.125708 ABIN967755 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
0.125708 ABIN2854697 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.125708 ABIN499082 WB Rabbit Log in to see Polyclonal
0.125708 ABIN257708 IHC (p) IHC ELISA WB Goat IgG C-Term, AA 206-217 Log in to see Polyclonal
0.125708 ABIN189380 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y237
0.125708 ABIN1872885 WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN1876890 IHC WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN2467826 WB Chicken AA 1-217 Log in to see Polyclonal
0.125708 ABIN2440123 ELISA WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN3184932 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.125708 ABIN2406480 IHC WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN2435838 IHC WB Goat Log in to see Polyclonal
0.125708 ABIN1847698 IHC ELISA WB Rabbit Internal Region, pSer159, pSer160 Log in to see Polyclonal
0.001176447 ABIN782889 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal 1
0.001176447 ABIN374150 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal 1
0.001176447 ABIN264897 WB Rabbit IgG C-Term, AA 198-217 Log in to see Polyclonal 1

Top referenced anti-GRB2 antibody for Western Blotting

Similar anti-GRB2 Antibodies

Application / Reactivity Mouse (Murine) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 16 Antibodies 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 6 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 56 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 41 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(34), (18), (15), (7), (6), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(148), (42), (41), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1)
Host Goat
(99), (29), (21), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(5), (4), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(129), (64), (52), (30), (9), (8), (5), (3), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN184670) Images
Blocking Peptide Blocking peptide for this product available: ABIN369579
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 Antibody (ABIN184670) Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Synonyms: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885, 14784, 81504
NCBI Accession NP_987102, NP_002077
Research Area Cancer
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 Antibody (ABIN184670) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in Jurkat and NIH3T3 lysates. Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration: 0.1-0.3 µg/mL. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained (please contact technical support for details).
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 Antibody (ABIN184670) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-GRB2 Antibody (ABIN184670)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN184670) back to top
Supplier Images
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN184670) ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN184670) ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...