anti-Rat (Rattus) GRB2 antibody for Western Blotting

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
N-Term
34
17
16
7
6
6
6
5
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
158
64
64
17
10
9
7
5
5
4
3
3
3
3
3
2
2
1
1
Host
Mouse
105
27
25
2
Clonality
Monoclonal
Conjugate
This GRB2 antibody is un-conjugated
8
4
3
3
3
3
Application
Western Blotting (WB)
141
69
55
31
13
7
5
3
2
2
2
1
Supplier
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Catalog No. ABIN2853618
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN1532105 IHC ELISA WB Rabbit IgG pSer159 Log in to see Polyclonal 2
3.3487515 ABIN1534949 ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.3487515 ABIN967755 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
3.3487515 ABIN967754 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
3.3487515 ABIN257708 ELISA IHC IHC (p) WB Goat C-Term, both Isoforms Log in to see Polyclonal 1
3.3487515 ABIN3044446 WB Rabbit IgG AA 184-201, C-Term Log in to see Polyclonal
3.3487515 ABIN2787701 WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2854311 IHC (p) WB Rabbit IgG full length Log in to see Polyclonal
3.3487515 ABIN3184932 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3487515 ABIN499082 WB Rabbit Log in to see Polyclonal
3.3487515 ABIN1872885 IF WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN2467826 WB Chicken AA 1-217 Log in to see Polyclonal
3.3487515 ABIN1876890 IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN4902647 ELISA WB Biotin Goat C-Term Log in to see Polyclonal
3.3487515 ABIN4316134 WB Rabbit Log in to see Polyclonal
3.3487515 ABIN2562931 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal
3.3487515 ABIN2406480 IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN3031113 WB Rabbit IgG C-Term Log in to see Polyclonal
3.3487515 ABIN1847698 IHC ELISA WB Rabbit Internal Region, pSer159, pSer160 Log in to see Polyclonal
1 ABIN264897 WB Rabbit IgG C-Term, AA 198-217 Log in to see Polyclonal 1

Top referenced anti-GRB2 antibody for Western Blotting

Similar anti-GRB2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 141 Antibodies 57 Antibodies 57 Antibodies
Immunoprecipitation (IP) 7 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies 14 Antibodies 14 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 14 Antibodies 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope N-Term
(34), (17), (16), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(158), (64), (64), (17), (10), (9), (7), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(105), (27), (25), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GRB2 antibody is un-conjugated
(8), (4), (3), (3), (3), (3)
Application Western Blotting (WB)
(141), (69), (55), (31), (13), (7), (5), (3), (2), (2), (2), (1)
Pubmed 6 references available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN2853618) Images
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat
Purification Ammonium sulfate precipitation and affinity chromatography on protein A
Immunogen Recombinant Ash/Grb-2
Clone 3F2
Isotype IgG1

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN2853618) Images back to top
Antigen
Alternative Name GRB2 / ASH (GRB2 Antibody Abstract)
Background Grb2 (Growth factor receptor bound protein 2) also known as Ash, is a 25-28 kDa molecule composed of central Src homology (SH2) domain and SH3 domains. Grb2 associates with tyrosine phosphorylated proteins such as EGFR, PDGFR, IRS-1, Shc and Gab1 via its Sh2 domain. The SH3 domain of Grb2 binds to Sos, which stimulates the GTP binding activity of Ras, cause activation of MAP kinase and other signaling pathways. Grb2 is an adaptor protein, which does not have enzymatic activity itself. Grb2 express ubiquitously and has two isoforms.Synonyms: Growth factor receptor-bound protein 2
Gene ID 2885
NCBI Accession NP_002077
UniProt P62993
Research Area Signaling
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling ProductDetails: References for anti-GRB2 antibody (ABIN2853618) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details ProductDetails: References for anti-GRB2 antibody (ABIN2853618) Images back to top
Reconstitution Restore with 100 μL of distilled water.
Buffer PBS, 1 mg/mL Sodium azide, 10 mg/mL sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted (in aliquots) at -20 °C.

ProductDetails: References for anti-GRB2 antibody (ABIN2853618)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Rozakis-Adcock, Fernley, Wade, Pawson, Bowtell: "The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1." in: Nature, Vol. 363, Issue 6424, pp. 83-5, 1993

Li, Batzer, Daly, Yajnik, Skolnik, Chardin, Bar-Sagi, Margolis, Schlessinger: "Guanine-nucleotide-releasing factor hSos1 binds to Grb2 and links receptor tyrosine kinases to Ras signalling." in: Nature, Vol. 363, Issue 6424, pp. 85-8, 1993

Gale, Kaplan, Lowenstein, Schlessinger, Bar-Sagi: "Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras." in: Nature, Vol. 363, Issue 6424, pp. 88-92, 1993

Matuoka, Shibata, Yamakawa, Takenawa: "Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 9015-9, 1992

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Clark, Stern, Horvitz: "C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains." in: Nature, Vol. 356, Issue 6367, pp. 340-4, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN2853618) back to top
Supplier Images
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (N-Term) (ABIN2853618) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (N-Term) antibody