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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||129 Antibodies||41 Antibodies||40 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||32 Antibodies||9 Antibodies||9 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies||15 Antibodies||13 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||11 Antibodies||6 Antibodies||5 Antibodies|
|Antigen||Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GRB2 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-GRB2 AntibodyTarget Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN2853618) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat|
|Purification||Ammonium sulfate precipitation and affinity chromatography on protein A|
Target Details GRB2Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 Antibody (ABIN2853618) Images back to top
|Alternative Name||GRB2 / ASH (GRB2 Antibody Abstract)|
|Background||Grb2 (Growth factor receptor bound protein 2) also known as Ash, is a 25-28 kDa molecule composed of central Src homology (SH2) domain and SH3 domains. Grb2 associates with tyrosine phosphorylated proteins such as EGFR, PDGFR, IRS-1, Shc and Gab1 via its Sh2 domain. The SH3 domain of Grb2 binds to Sos, which stimulates the GTP binding activity of Ras, cause activation of MAP kinase and other signaling pathways. Grb2 is an adaptor protein, which does not have enzymatic activity itself. Grb2 express ubiquitously and has two isoforms.Synonyms: Growth factor receptor-bound protein 2|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 Antibody (ABIN2853618) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 Antibody (ABIN2853618) Images back to top
|Reconstitution||Restore with 100 μL of distilled water.|
|Buffer||PBS, 1 mg/mL Sodium azide, 10 mg/mL sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted (in aliquots) at -20 °C.|
References for anti-GRB2 Antibody (ABIN2853618)Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Li, Batzer, Daly et al.: "Guanine-nucleotide-releasing factor hSos1 binds to Grb2 and links receptor tyrosine kinases to Ras signalling." in: Nature, Vol. 363, Issue 6424, pp. 85-8, 1993 (PubMed).
Rozakis-Adcock, Fernley, Wade et al.: "The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1." in: Nature, Vol. 363, Issue 6424, pp. 83-5, 1993 (PubMed).
Gale, Kaplan, Lowenstein et al.: "Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras." in: Nature, Vol. 363, Issue 6424, pp. 88-92, 1993 (PubMed).
Matuoka, Shibata, Yamakawa et al.: "Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 9015-9, 1992 (PubMed).
Clark, Stern, Horvitz: "C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains." in: Nature, Vol. 356, Issue 6367, pp. 340-4, 1992 (PubMed).
Lowenstein, Daly, Batzer et al.: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992 (PubMed).
ImagesProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN2853618) back to top