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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Xenopus laevis|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||138 Antibodies||56 Antibodies||56 Antibodies||14 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||30 Antibodies||9 Antibodies||14 Antibodies||14 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||55 Antibodies||14 Antibodies||13 Antibodies||1 Antibodies|
|Antigen||Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This GRB2 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-GRB2 AntibodyTarget Details GRB2 Application Details Handling Images
|Specificity||This antibody detects GRB2 at aa 54-62|
|Characteristics||Synonyms: Growth factor receptor-bound protein 2|
|Purification||Protein G chromatography|
|Immunogen||Purified recombinant human Grb2 protein|
Target Details GRB2Product Details anti-GRB2 Antibody Application Details Handling Images back to top
|Alternative Name||GRB2 / ASH (GRB2 Antibody Abstract)|
|Background||Grb2 protein contains one SH2 domain flanked on both sides by an SH3 domain. It binds to the junction between the amino terminal SH3 domain and the SH2 domain of Grb2. Reactivity with Grb2 deletion mutants suggests that its epitope maps between aa 54-62. In response to EGF, Grb2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The Grb2-Sos complex then binds the EGF-receptor. These interactions play a critical role in the activation of the p21ras signalling pathway.Synonyms: Growth factor receptor-bound protein 2|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation|
Application DetailsProduct Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Immunofluorescence. Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HeLa cells. Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
|Buffer||10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top