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anti-Human HGF antibody for Immunohistochemistry

Recommended HGF Antibody (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
  • HGF
  • C230052L06Rik
  • HGF/SF
  • NK1
  • NK2
  • SF/HGF
  • HPTA
  • DFNB39
  • F-TCF
  • HGFB
  • SF
  • AI838057
  • HGFR
  • Par4
  • c-Met
  • hepatocyte growth factor
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • hepatocyte growth factor-like
  • met proto-oncogene
  • HGF
  • Hgf
  • LOC100525120
  • Met
Epitope
N-Term
22
19
15
14
14
10
8
7
6
5
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
214
53
50
10
3
3
2
2
2
2
2
1
1
1
Host
Rabbit
138
95
4
4
Clonality
Polyclonal
Conjugate
This HGF antibody is un-conjugated
18
14
6
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
194
117
73
27
16
13
13
12
7
5
5
4
2
2
1
1
1
1
1
Options
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Catalog No. ABIN2781816
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.583081 ABIN2781817 IHC WB Rabbit N-Term Log in to see Polyclonal 1
8.583081 ABIN2469921 IHC WB Mouse IgG2a kappa Log in to see 07-02-15
8.583081 ABIN1584562 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
8.583081 ABIN4317238 IHC WB Mouse IgG2a Log in to see 1D2
8.583081 ABIN3031186 FACS IHC ELISA WB Rabbit Ig Fraction AA 521-554 Log in to see Polyclonal
8.583081 ABIN4317240 IHC Rabbit IgG Log in to see Polyclonal
8.583081 ABIN4317239 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
8.583081 ABIN2428213 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.583081 ABIN2421657 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN438999 IHC (p) IHC WB Mouse IgG2a Log in to see 7-2 1
1 ABIN251141 ELISA IHC WB Mouse IgG1 Log in to see SBF5 C1-7 1
1 ABIN1872965 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3021683 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4891902 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1824715 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1824717 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1824718 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1859145 ICC IHC IP WB Rabbit IgG AA 32-291 Log in to see Polyclonal
1 ABIN1859146 ICC IHC IP WB Rabbit IgG AA 495-728 Log in to see Polyclonal
1 ABIN1078141 ICC IHC IP WB Rabbit IgG AA 495-728 Log in to see Polyclonal

Top referenced anti-HGF antibody for Immunohistochemistry

Similar anti-HGF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Cow (Bovine) Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 171 Antibodies 4 Antibodies 11 Antibodies 37 Antibodies 4 Antibodies 33 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 5 Antibodies
Immunoprecipitation (IP) 9 Antibodies 5 Antibodies 5 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies 11 Antibodies 6 Antibodies 1 Antibodies

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
Epitope N-Term
(22), (19), (15), (14), (14), (10), (8), (7), (6), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(214), (53), (50), (10), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(138), (95), (4), (4)
Clonality
Polyclonal
Conjugate This HGF antibody is un-conjugated
(18), (14), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(194), (117), (73), (27), (16), (13), (13), (12), (7), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-HGF Antibody

Target Details HGF Application Details Handling References for anti-HGF Antibody (ABIN2781816) Images
Blocking Peptide Blocking peptide for this product available: ABIN977963
Sequence VKKEFGHEFD LYENKDYIRN CIIGKGRSYK GTVSITKSGI KCQPWSSMIP
Specificity 100 % homologous to all 6 isoforms, 1 (83 kDa), 2 (34 kDa), 3 (83 kDa), 4 (35 kDa), 5 (33 kDa), 6 (24 kDa)
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Sheep : 100 %, Guinea pig : 90 %
Characteristics This is a rabbit polyclonal antibody against HGF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HGF

Target Details HGF

Product Details anti-HGF Antibody Application Details Handling References for anti-HGF Antibody (ABIN2781816) Images back to top
Antigen
Alternative Name HGF (HGF Antibody Abstract)
Background Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity.Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
Molecular Weight 83 kDa
Gene ID 3082
NCBI Accession NM_001010932, NP_001010932
UniProt P14210
Research Area Cancer, Angiogenesis, Hormones, Growth Factors
Pathways RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-HGF Antibody Target Details HGF Handling References for anti-HGF Antibody (ABIN2781816) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 728 AA

Restrictions For Research Use only

Handling

Product Details anti-HGF Antibody Target Details HGF Application Details References for anti-HGF Antibody (ABIN2781816) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HGF Antibody (ABIN2781816)

Product Details anti-HGF Antibody Target Details HGF Application Details Handling Images back to top
Product cited in:

Nejak-Bowen, Orr, Bowen, Michalopoulos: "Conditional genetic elimination of hepatocyte growth factor in mice compromises liver regeneration after partial hepatectomy." in: PLoS ONE, Vol. 8, Issue 3, pp. e59836, 2013

Ji, Zhang, You, Li, Liu, Jiang, Liu, Zhang, Wang, Tao, Dou: "The differentiation of MSCs into functional hepatocyte-like cells in a liver biomatrix scaffold and their transplantation into liver-fibrotic mice." in: Biomaterials, Vol. 33, Issue 35, pp. 8995-9008, 2012

Zampell, Avraham, Yoder, Fort, Yan, Weitman, Mehrara: "Lymphatic function is regulated by a coordinated expression of lymphangiogenic and anti-lymphangiogenic cytokines." in: American journal of physiology. Cell physiology, Vol. 302, Issue 2, pp. C392-404, 2012

Sen, Peng, Saigal, Williams, Johnson: "Distinct interactions between c-Src and c-Met in mediating resistance to c-Src inhibition in head and neck cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 3, pp. 514-24, 2011

Williams, Mehta, Peyton, Zeiger, Van Vliet, Griffith: "Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays." in: Tissue engineering. Part A, Vol. 17, Issue 7-8, pp. 1055-68, 2011

Background publications

Kitajima, Ide, Ohtsuka, Miyazaki: "Induction of hepatocyte growth factor activator gene expression under hypoxia activates the hepatocyte growth factor/c-Met system via hypoxia inducible factor-1 in pancreatic cancer." in: Cancer science, Vol. 99, Issue 7, pp. 1341-7, 2008

Images

Product Details anti-HGF Antibody Target Details HGF Application Details Handling References for anti-HGF Antibody (ABIN2781816) back to top
Supplier Images
Western Blotting (WB) image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (N-Term) (ABIN2781816) WB Suggested Anti-HGF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
Immunohistochemistry (IHC) image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (N-Term) (ABIN2781816) anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (N-Term) (ABIN2781816) Host: Rabbit
Target Name: HGF
Sample Tissue: Human HepG2 cell
La...