HGF antibody (AA 216-265)

Catalog No. ABIN604744

This Rabbit Polyclonal antibody specifically detects HGF in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Guinea Pig, Hamster and Monkey.

Quick Overview for HGF antibody (AA 216-265) (ABIN604744)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Guinea Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HGF Antibody

Binding Specificity: AA 216-265

Specificity: Human HGF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human HGF (P14210, NP_000592). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Bat (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: HGF

Background: Name/Gene ID: HGF
Subfamily: Serine S1
Family: Protease

Synonyms: HGF, DFNB39, Hepatocyte growth factor, Hepatopoeitin-A, Hepapoietin A, Hepatopoietin-A, Scatter factor), SF, F-TCF, HGFB, HPTA, Scatter factor

Gene ID: 3082

NCBI Accession: NP_000592

UniProt: P14210

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor