|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Chicken|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||3 Antibodies||3 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||206 Antibodies||136 Antibodies||130 Antibodies||8 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies||2 Antibodies||4 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||25 Antibodies||14 Antibodies||13 Antibodies|
|Antigen||phospholipase C, gamma 1 (PLCG1) Antibodies|
|Epitope||AA 82-100 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Phospholipase C gamma 1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-Phospholipase C gamma 1 AntibodyTarget Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Cow PLCgamma1 aa. 82-100|
Target Details Phospholipase C gamma 1Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
|Alternative Name||Phospholipase C gamma 1 (PLCG1 Antibody Abstract)|
The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCg activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCg1, regardless of phosphorylation status. It does not cross-react with PLCg2.
|Molecular Weight||148 kDa|
|Pathways||RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
Related Products: ABIN968533, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-Phospholipase C gamma 1 Antibody (ABIN967699)Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling Images back to top
|Product cited in:||
Vossmeyer, Hofmann, Löster, Reutter, Danker: "Phospholipase Cgamma binds alpha1beta1 integrin and modulates alpha1beta1 integrin-specific adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4636-43, 2002
Dayanir, Meyer, Lashkari, Rahimi: "Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17686-92, 2001
Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, 2000
Chen, Murphy-Ullrich, Wells: "A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility." in: The Journal of cell biology, Vol. 134, Issue 3, pp. 689-98, 1996
Obermeier, Tinhofer, Grunicke, Ullrich: "Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation." in: The EMBO journal, Vol. 15, Issue 1, pp. 73-82, 1996
ImagesProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) back to top