You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Dog (Canine) Phospholipase C gamma 1 antibody for Immunohistochemistry

Recommended Phospholipase C gamma 1 Antibody (supplied by: Log in to see )

Antigen
phospholipase C, gamma 1 (PLCG1) Antibodies
  • AI894140
  • Cded
  • nckap3
  • NCKAP3
  • Phosphb
  • PLC'1
  • Plc-1
  • Plc-gamma1
  • PLC-II
  • plc-ii
  • plc1
  • PLC1
  • plc148
  • PLC148
  • PLCbeta1
  • Plcg-1
  • plcgamma1
  • PLCgamma1
  • PPLCA
  • RATPHOSPHB
Epitope
N-Term
50
41
13
13
9
9
8
8
6
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
216
131
124
11
8
4
3
1
Host
Mouse
192
28
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
3
Application
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
157
56
49
34
31
27
25
6
4
4
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967698
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011407726 ABIN967699 IF IP IHC WB Mouse IgG1 AA 82-100 Log in to see 10-PLCgamma 5

Top referenced anti-Phospholipase C gamma 1 antibody for Immunohistochemistry

Similar anti-Phospholipase C gamma 1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken
Western Blotting (WB) 155 Antibodies 82 Antibodies 76 Antibodies 3 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 22 Antibodies 4 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies 16 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 57 Antibodies

General

Antigen phospholipase C, gamma 1 (PLCG1) Antibodies
Epitope N-Term
(50), (41), (13), (13), (9), (9), (8), (8), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(216), (131), (124), (11), (8), (4), (3), (1)
Host Mouse
(192), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(157), (56), (49), (34), (31), (27), (25), (6), (4), (4), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-Phospholipase C gamma 1 Antibody

Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967698) Images
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
5. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Cow PLCgamma1 N-terminal region Peptide
Clone 10-PLCgamma
Isotype IgG1

Target Details Phospholipase C gamma 1

Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967698) Images back to top
Antigen
Alternative Name Phospholipase C gamma 1 (PLCG1 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCg activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCg1, regardless of phosphorylation status. It does not cross-react with PLCg2.
Molecular Weight 148 kDa
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967698) Images back to top
Application Notes Recommended Protocol for Bioimaging:
1. Seed the cells in appropriate culture medium at an appropriate cell density in an 96-well Imaging Plate , and culture overnight to 48 hours.
2. Remove the culture medium from the wells, and wash (one to two times) with 100 µl of 1× PBS.
3. Fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or fixation buffer to each well and incubating for 10 minutes at room temperature (RT).
4. Remove the fixative from the wells, and wash the wells (one to two times) with 100 µl of 1× PBS.
5. Permeabilize the cells using either cold methanol (a), Triton™ X-100 (b), or Saponin (c): a. Add 100 µl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. b. Add 100 µl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT. c. Add 100 µl of 1× Perm/Wash buffer to each well and incubate for 15 to 30 minutes at RT. Continue to use 1× Perm/Wash buffer for all subsequent wash and dilutions steps.
6. Remove the permeabilization buffer from the wells, and wash one to two times with 100 µl of appropriate buffer (either 1× PBS or 1× Perm/Wash buffer, see step 5.c.).
7. Optional blocking step: Remove the wash buffers, and block the cells by adding 100 µl of blocking buffer or 3% FBS in appropriate dilution buffer to each well and incubating for 15 to 30 minutes at RT.
8. Dilute the antibody to its optimal working concentration in appropriate dilution buffer. Titrate purified (unconjugated) antibodies and second-step reagents to determine the optimal concentration. If using a Bioimaging Certified antibody conjugate, dilute it 1:10.
9. Add 50 µl of diluted antibody per well and incubate for 120 minutes at RT. Incubate in the dark if using fluorescently labeled antibodies.
10. Remove the antibody, and wash the wells three times with 100 µl of wash buffer. An optional detergent wash (100 µl of 0.05% Tween in 1× PBS) can be included prior to the regular wash steps.
11. If the antibody being used is fluorescently labeled, then move to step 12. Otherwise, if using a purified unlabeled antibody, repeat steps 8 to 10 with a fluorescently labeled second-step reagent to detect the purified antibody.
12. After the final wash, counter-stain the nuclei by adding 100 µl of a 2 µg/ml solution of Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
13. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details References for anti-Phospholipase C gamma 1 Antibody (ABIN967698) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Phospholipase C gamma 1 Antibody (ABIN967698)

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling Images back to top
Product cited in:

Vossmeyer, Hofmann, Löster, Reutter, Danker: "Phospholipase Cgamma binds alpha1beta1 integrin and modulates alpha1beta1 integrin-specific adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4636-43, 2002

Dayanir, Meyer, Lashkari, Rahimi: "Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17686-92, 2001

Obermeier, Tinhofer, Grunicke, Ullrich: "Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation." in: The EMBO journal, Vol. 15, Issue 1, pp. 73-82, 1996

Images

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967698) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (N-Term) (ABIN967698) Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate (Human epithelia...
Immunofluorescence (IF) image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (N-Term) (ABIN967698) Immunofluorescence staining of HeLa cells. Cells were seeded in a 96 well imaging pla...